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Literature summary for 6.3.4.6 extracted from
- Enzymatic characterization of a prokaryotic urea carboxylase (2004), J. Bacteriol., 186, 2532-2539.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Oleomonas sagaranensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.17 | - |
Urea | 30°C, pH 7.5 | Oleomonas sagaranensis |  |
| 56.5 | - |
Formamide | 30°C, pH 7.5 | Oleomonas sagaranensis | |
| 126 | - |
acetamide | 30°C, pH 7.5 | Oleomonas sagaranensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | divalent cation required, Mg2+, Mn2+ or Co2+ support activity | Oleomonas sagaranensis | |
| Mg2+ | divalent cation is required for activity. Increase in activity at concentrations up to 4 mM Mg2+, higher concentrations do not inhibit | Oleomonas sagaranensis | |
| Mn2+ | divalent cation required, Mg2+, Mn2+ or Co2+ support activity | Oleomonas sagaranensis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + urea + CO2 | Oleomonas sagaranensis | activity is inducible by urea, acetamide or formamide | ADP + phosphate + urea-1-carboxylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oleomonas sagaranensis | Q75RY4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Oleomonas sagaranensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Oleomonas sagaranensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetamide + HCO3- | - |
Oleomonas sagaranensis | ? | - |
? | |
| ATP + formamide + HCO3- | - |
Oleomonas sagaranensis | ? | - |
? | |
| ATP + urea + CO2 | activity is inducible by urea, acetamide or formamide | Oleomonas sagaranensis | ADP + phosphate + urea-1-carboxylate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UCA | - |
Oleomonas sagaranensis |
| urea carboxylase | - |
Oleomonas sagaranensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16.8 | - |
Formamide | 30°C, pH 7.5 | Oleomonas sagaranensis | |
| 40.4 | - |
acetamide | 30°C, pH 7.5 | Oleomonas sagaranensis | |
| 44.2 | - |
Urea | 30°C, pH 7.5 | Oleomonas sagaranensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | HEPES-NaOH buffer | Oleomonas sagaranensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | pH 6.5: about 80% of maximal activity, pH 8.5: about 45% of maximal activity, Hepes-NaOH buffer | Oleomonas sagaranensis |
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Release 2023.1 (January 2023)
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