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Literature summary for 6.3.4.6 extracted from

  • Kimura, S.; Iyama, S.; Yamaguchi, Y.; Kanakura, Y.
    New enzymatic assay for serum urea nitrogen using urea amidolyase (2003), J. Clin. Lab. Anal., 17, 52-56.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.4.6

Activating Compound

Activating Compound Comment Organism Structure
ATP ATP-dependent cleavage of urea Saccharomyces cerevisiae

Application

Application Comment Organism
diagnostics enzymatic assay for measurement of serum urea nitrogen using urea amidolyase Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ strong, Ca2+ affects the enzyme-ATP-Mg complex by the time of urea carboxylation Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
HCO3- requirement, hydrolysis of urea Saccharomyces cerevisiae
K+ requirement, hydrolysis of urea Saccharomyces cerevisiae
Mg2+ requirement, hydrolysis of urea Saccharomyces cerevisiae
additional information requires divalent and monovalent ions Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P32528
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + urea + HCO3- + H+ = ADP + phosphate + urea-1-carboxylate a biotinyl-protein, the yeast enzyme (but not that from green algae) also catalyses the reaction of EC 3.5.1.54 allophanate hydrolase, thus bringing about the hydrolysis of urea to CO2 and NH3, previously also listed as EC 3.5.1.45 Saccharomyces cerevisiae
a

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + urea + CO2 multifunctional enzyme, catalyzes carboxylation of urea with urea carboxylase, EC 6.3.4.6, and hydrolysis of allophanate with allophanate hydrolase, EC 3.5.1.54 Saccharomyces cerevisiae ADP + phosphate + urea-1-carboxylate
-
 ?
urea + H2O + ATP in the presence of enzyme, ATP, bicarbonate, magnesium, and potassium ions, ammonium ion is produced from human serum urea Saccharomyces cerevisiae CO2 + NH3 + ADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
ATP-urea amidolyase
-
 Saccharomyces cerevisiae
urea amidolyase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 hydrolysis of urea Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information
-
 Saccharomyces cerevisiae