Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Cryptococcus neoformans |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M bis-Tris propane pH 8, 0.2 M sodium bromide, 17% (w/v) polyethylene glycol 3350 | Cryptococcus neoformans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Cryptococcus neoformans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
2 * 46000, SDS-PAGE | Cryptococcus neoformans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cryptococcus neoformans | - |
Cryptococcus neoformans variant grubii | - |
Cryptococcus neoformans H99 | - |
Cryptococcus neoformans variant grubii | - |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 200 gel filtration | Cryptococcus neoformans |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 46000, SDS-PAGE | Cryptococcus neoformans |
Synonyms | Comment | Organism |
---|---|---|
Adenylosuccinate synthetase | - |
Cryptococcus neoformans |
AdSS | - |
Cryptococcus neoformans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
GTP | - |
Cryptococcus neoformans |