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Literature summary for 6.3.4.4 extracted from

  • Blundell, R.D.; Williams, S.J.; Morrow, C.A.; Ericsson, D.J.; Kobe, B.; Fraser, J.A.
    Purification, crystallization and preliminary X-ray analysis of adenylosuccinate synthetase from the fungal pathogen Cryptococcus neoformans (2013), Acta Crystallogr. Sect. F, 69, 1033-1036.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Cryptococcus neoformans

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M bis-Tris propane pH 8, 0.2 M sodium bromide, 17% (w/v) polyethylene glycol 3350 Cryptococcus neoformans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Cryptococcus neoformans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
2 * 46000, SDS-PAGE Cryptococcus neoformans

Organism

Organism UniProt Comment Textmining
Cryptococcus neoformans
-
Cryptococcus neoformans variant grubii
-
Cryptococcus neoformans H99
-
Cryptococcus neoformans variant grubii
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 200 gel filtration Cryptococcus neoformans

Subunits

Subunits Comment Organism
homodimer 2 * 46000, SDS-PAGE Cryptococcus neoformans

Synonyms

Synonyms Comment Organism
Adenylosuccinate synthetase
-
Cryptococcus neoformans
AdSS
-
Cryptococcus neoformans

Cofactor

Cofactor Comment Organism Structure
GTP
-
Cryptococcus neoformans