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Literature summary for 6.3.4.4 extracted from

  • Iancu, C.V.; Borza, T.; Choe, J.Y.; Fromm, H.J.; Honzatko, R.B.
    Recombinant mouse muscle adenylosuccinate synthetase: overexpression, kinetics, and crystal structure (2001), J. Biol. Chem., 276, 42146-42152.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli BL21 (DE3) Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals grown by the method of hanging drops, space group P4(#)2(1)2, a = b = 69.93, c = 198.49 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
GTP pH 7.2, 22°C Mus musculus
0.045
-
IMP pH 7.2, 22°C Mus musculus
0.14
-
L-aspartate pH 7.2, 22°C Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + IMP + L-aspartate Mus musculus catalyzes the first committed step in the de novo biosynthesis of AMP GDP + phosphate + adenylosuccinate
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
mouse
-

Purification (Commentary)

Purification (Comment) Organism
-
Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + IMP + L-aspartate
-
Mus musculus GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate catalyzes the first committed step in the de novo biosynthesis of AMP Mus musculus GDP + phosphate + adenylosuccinate
-
?
additional information vertebrates possess two isozymes, the acidic is similar to the synthetase from bacteria and plants, the basic isozyme participates in the purine nucleotide cycle Mus musculus ?
-
?

Synonyms

Synonyms Comment Organism
Adenylosuccinate synthetase
-
Mus musculus
mouse muscle synthetase
-
Mus musculus