Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Rattus norvegicus | |
AMP | - |
Rattus norvegicus | |
Ca2+ | in presence of Mg2+ | Rattus norvegicus | |
Cd2+ | in presence of Mg2+ | Rattus norvegicus | |
Cu2+ | in presence of Mg2+ | Rattus norvegicus | |
dGTP | - |
Rattus norvegicus | |
GDP | - |
Rattus norvegicus | |
GMP | - |
Rattus norvegicus | |
guanosine | - |
Rattus norvegicus | |
Hadacidin | - |
Rattus norvegicus | |
Hg2+ | reversed by addition of DTT | Rattus norvegicus | |
IDP | - |
Rattus norvegicus | |
Mn2+ | in presence of Mg2+ | Rattus norvegicus | |
Pb2+ | in presence of Mg2+ | Rattus norvegicus | |
PCMB | - |
Rattus norvegicus | |
XMP | - |
Rattus norvegicus | |
Zn2+ | in presence of Mg2+ | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
GTP | - |
Rattus norvegicus | |
0.41 | - |
IMP | - |
Rattus norvegicus | |
0.98 | - |
L-Asp | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 14.1% of the activation relative to Mg2+ | Rattus norvegicus | |
Co2+ | 34.8% of the activation relative to Mg2+ | Rattus norvegicus | |
Cu2+ | 10.9% of the activation relative to Mg2+ | Rattus norvegicus | |
Mg2+ | full activity in presence of Mg2+ | Rattus norvegicus | |
Mn2+ | 37% of the activation relative to Mg2+ | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
2 * 47000, sedimentation equilibrium analysis in presence of 6 M guanidine-HCl | Rattus norvegicus |
102000 | - |
Yoshida sarcoma ascites tumor cells, sedimentation equilibrium analysis | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Yoshida ascites sarcoma cell | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.36 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, or 0°C, rapid loss of enzyme activity in dilute solution | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + IMP + L-Asp | - |
Rattus norvegicus | GDP + phosphate + adenylosuccinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 47000, sedimentation equilibrium analysis in presence of 6 M guanidine-HCl | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7 | - |
Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
most stable at pH 7.0 in phosphate buffer | Rattus norvegicus |