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Literature summary for 6.3.4.3 extracted from

  • Prasannan, P.; Appling, D.R.
    Human mitochondrial C(1)-tetrahydrofolate synthase: Submitochondrial localization of the full-length enzyme and characterization of a short isoform (2008), Arch. Biochem. Biophys., 481, 86-93.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in CHO cells and Escherichia coli Homo sapiens
expressed in CHO cells and Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information the recombinant protein product (short isoform) of the alternatively spliced short transcript of the mitochondrial isozyme is not enzymatically active Homo sapiens
additional information the recombinant protein product (short isoform) of the alternatively spliced short transcript of the mitochondrial isozyme is not enzymatically active Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion mitochondrial C1-THF synthase behaves as a peripheral membrane protein, tightly associated with the matrix side of the mitochondrial inner membrane Homo sapiens 5739
-
mitochondrion mitochondrial C1-THF synthase behaves as a peripheral membrane protein, tightly associated with the matrix side of the mitochondrial inner membrane Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
recombinant short isoform, SDS-PAGE Homo sapiens
107000
-
recombinant mitochondrial C1-THF synthase without the presequence but with the V5 epitope tag, SDS-PAGE Homo sapiens
107000
-
recombinant mitochondrial C1-THF synthase without the presequence but with the V5 epitope tag, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
TALON cobalt metal affinity resin chromatography Homo sapiens
TALON cobalt metal affinity resin chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
Homo sapiens
-
spleen
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + formate + tetrahydrofolate
-
Homo sapiens ADP + phosphate + 10-formyltetrahydrofolate
-
?
ATP + formate + tetrahydrofolate
-
Rattus norvegicus ADP + phosphate + 10-formyltetrahydrofolate
-
?

Synonyms

Synonyms Comment Organism
C(1)-tetrahydrofolate synthase a monofunctional 10-formyl-tetrahydrofolate synthetase lacking the 5,10-methylene-tetrahydrofolate dehydrogenase and 5,10-methenyl-tetrahydrofolate cyclohydrolase activities typically found in the trifunctional cytoplasmic proteins Homo sapiens
C(1)-tetrahydrofolate synthase a monofunctional 10-formyl-tetrahydrofolate synthetase lacking the 5,10-methylene-tetrahydrofolate dehydrogenase and 5,10-methenyl-tetrahydrofolate cyclohydrolase activities typically found in the trifunctional cytoplasmic proteins Rattus norvegicus
MTHFDIL
-
Homo sapiens
MTHFDIL
-
Rattus norvegicus
THF synthase
-
Homo sapiens
THF synthase
-
Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens
ATP
-
Rattus norvegicus