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Literature summary for 6.3.4.3 extracted from

  • Walkup, A.S.; Appling, D.R.
    Enzymatic characterization of human mitochondrial C1-tetrahydrofolate synthase (2005), Arch. Biochem. Biophys., 442, 196-205.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of the mitochondrial isozyme as maltose-binding protein fusion protein in Escherichia coli, overexpression in yeast Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics, recombinant enzyme Homo sapiens
0.0036
-
(6R,S)-tetrahydrofolate pteroylpentaglutamate recombinant enzyme, 30°C Homo sapiens
0.016
-
(6R,S)-tetrahydrofolate pteroyltriglutamate recombinant enzyme, 30°C Homo sapiens
0.04
-
ATP recombinant enzyme, 30°C, with substrate (6R,S)-tetrahydrofolate pteroyltriglutamate or (6R,S)-tetrahydrofolate pteroylpentaglutamate and formate Homo sapiens
0.15
-
formate recombinant enzyme, 30°C, with substrate (6R,S)-tetrahydrofolate pteroylpentaglutamate Homo sapiens
0.2
-
ATP above, recombinant enzyme, 30°C, with substrate (6R,S)-tetrahydrofolate monoglutamate and formate Homo sapiens
0.44
-
formate recombinant enzyme, 30°C, with substrate (6R,S)-tetrahydrofolate pteroyltriglutamate Homo sapiens
0.5
-
(6R,S)-tetrahydrofolate monoglutamate above, recombinant enzyme, 30°C Homo sapiens
11.8
-
formate recombinant enzyme, 30°C, with substrate (6R,S)-tetrahydrofolate monoglutamate Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion mitochondrial isozyme Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates at 100-200 mM, inhibitory above 200 mM Homo sapiens
Mg2+
-
Homo sapiens
NH4+ activates, maximal at 100 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
102000
-
2 * 102000, homodimer, sequence calculation Homo sapiens
143000
-
recombinant mitochondrial isozyme, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens enzyme is involved in the folate-mediated one-carbon metabolism, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant maltose-binding protein fusion mitochondrial isozyme from Escherichia coli by metal affinity chromatography and gel filtration, the MBP-tag is cleaved off by TEV protease Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate the enzyme is not trifunctional showing only 10-formyl-THS synthetase activity, enzyme evolutionary considerations Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (6R,S)-tetrahydrofolate pteroylpentaglutamate
-
Homo sapiens ADP + phosphate + 10-formyltetrahydrofolate pteroylpentaglutamate
-
?
ATP + (6R,S)-tetrahydrofolate pteroyltriglutamate
-
Homo sapiens ADP + phosphate + 10-formyltetrahydrofolate pteroyltriglutamate
-
?
ATP + formate + (6R,S)-tetrahydrofolate monoglutamate
-
Homo sapiens ADP + phosphate + 10-formyltetrahydrofolate monoglutamate
-
?
additional information enzyme is involved in the folate-mediated one-carbon metabolism, overview Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 102000, homodimer, sequence calculation Homo sapiens

Synonyms

Synonyms Comment Organism
10-formyl-THF synthetase
-
Homo sapiens
C1-tetrahydrofolate synthase
-
Homo sapiens
C1-THF synthase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6
-
(6R,S)-tetrahydrofolate pteroylpentaglutamate recombinant enzyme, 30°C Homo sapiens
10
-
(6R,S)-tetrahydrofolate pteroyltriglutamate recombinant enzyme, 30°C Homo sapiens
16
-
(6R,S)-tetrahydrofolate monoglutamate above, recombinant enzyme, 30°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens