Cloned (Comment) | Organism |
---|---|
expression of the mitochondrial isozyme as maltose-binding protein fusion protein in Escherichia coli, overexpression in yeast | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, recombinant enzyme | Homo sapiens | |
0.0036 | - |
(6R,S)-tetrahydrofolate pteroylpentaglutamate | recombinant enzyme, 30°C | Homo sapiens | |
0.016 | - |
(6R,S)-tetrahydrofolate pteroyltriglutamate | recombinant enzyme, 30°C | Homo sapiens | |
0.04 | - |
ATP | recombinant enzyme, 30°C, with substrate (6R,S)-tetrahydrofolate pteroyltriglutamate or (6R,S)-tetrahydrofolate pteroylpentaglutamate and formate | Homo sapiens | |
0.15 | - |
formate | recombinant enzyme, 30°C, with substrate (6R,S)-tetrahydrofolate pteroylpentaglutamate | Homo sapiens | |
0.2 | - |
ATP | above, recombinant enzyme, 30°C, with substrate (6R,S)-tetrahydrofolate monoglutamate and formate | Homo sapiens | |
0.44 | - |
formate | recombinant enzyme, 30°C, with substrate (6R,S)-tetrahydrofolate pteroyltriglutamate | Homo sapiens | |
0.5 | - |
(6R,S)-tetrahydrofolate monoglutamate | above, recombinant enzyme, 30°C | Homo sapiens | |
11.8 | - |
formate | recombinant enzyme, 30°C, with substrate (6R,S)-tetrahydrofolate monoglutamate | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | mitochondrial isozyme | Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates at 100-200 mM, inhibitory above 200 mM | Homo sapiens | |
Mg2+ | - |
Homo sapiens | |
NH4+ | activates, maximal at 100 mM | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
102000 | - |
2 * 102000, homodimer, sequence calculation | Homo sapiens |
143000 | - |
recombinant mitochondrial isozyme, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme is involved in the folate-mediated one-carbon metabolism, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant maltose-binding protein fusion mitochondrial isozyme from Escherichia coli by metal affinity chromatography and gel filtration, the MBP-tag is cleaved off by TEV protease | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate | the enzyme is not trifunctional showing only 10-formyl-THS synthetase activity, enzyme evolutionary considerations | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (6R,S)-tetrahydrofolate pteroylpentaglutamate | - |
Homo sapiens | ADP + phosphate + 10-formyltetrahydrofolate pteroylpentaglutamate | - |
? | |
ATP + (6R,S)-tetrahydrofolate pteroyltriglutamate | - |
Homo sapiens | ADP + phosphate + 10-formyltetrahydrofolate pteroyltriglutamate | - |
? | |
ATP + formate + (6R,S)-tetrahydrofolate monoglutamate | - |
Homo sapiens | ADP + phosphate + 10-formyltetrahydrofolate monoglutamate | - |
? | |
additional information | enzyme is involved in the folate-mediated one-carbon metabolism, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 102000, homodimer, sequence calculation | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
10-formyl-THF synthetase | - |
Homo sapiens |
C1-tetrahydrofolate synthase | - |
Homo sapiens |
C1-THF synthase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
(6R,S)-tetrahydrofolate pteroylpentaglutamate | recombinant enzyme, 30°C | Homo sapiens | |
10 | - |
(6R,S)-tetrahydrofolate pteroyltriglutamate | recombinant enzyme, 30°C | Homo sapiens | |
16 | - |
(6R,S)-tetrahydrofolate monoglutamate | above, recombinant enzyme, 30°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |