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Literature summary for 6.3.4.3 extracted from

  • Radfar, R.; Leaphart, A.; Brewer, J.M.; Minor, W.; Odom, J.D.; Dunlap, R.B.; Lovell, C.R.; Lebioda, L.
    Cation binding and thermostability of FTHFS monovalent cation binding sites and thermostability of N10-formyltetrahydrofolate synthetase from Moorella thermoacetica (2000), Biochemistry, 39, 14481-14486.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Moorella thermoacetica

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of FTHFS complexed with NH4+, K+ or Cs+ are grown by vapor diffusion from 46% saturated ammonium sulfate, 1 mM dithiothreitol and 1% polyehtylene glycol 1000 in 50 mM potassium maleate buffer, pH 7.6, crystals diffract to 3.0-3.2 A resolution Moorella thermoacetica

Protein Variants

Protein Variants Comment Organism
E98D 36% of wild-type activity Moorella thermoacetica
E98Q 93% of wild-type activity, slight increase in thermal stability in the absence of monovalent cation Moorella thermoacetica
E98S 61% of wild-type activity Moorella thermoacetica

Metals/Ions

Metals/Ions Comment Organism Structure
K+ monovalent cation required for activity Moorella thermoacetica
NH4+ monovalent cation required for activity Moorella thermoacetica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
4 * 60000, SDS-PAGE Moorella thermoacetica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + formate + tetrahydrofolate Moorella thermoacetica
-
ADP + phosphate + 10-formyltetrahydrofolate
-
?

Organism

Organism UniProt Comment Textmining
Moorella thermoacetica P21164
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant FTHFS Moorella thermoacetica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
780
-
recombinant FTHFS Moorella thermoacetica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + formate + tetrahydrofolate
-
Moorella thermoacetica ADP + phosphate + 10-formyltetrahydrofolate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 60000, SDS-PAGE Moorella thermoacetica

Synonyms

Synonyms Comment Organism
FTHFS
-
Moorella thermoacetica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55 60
-
Moorella thermoacetica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
69
-
in the absence of monovalent cation Moorella thermoacetica
79
-
in the presence of 200 mM K+ Moorella thermoacetica