Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Moorella thermoacetica |
Crystallization (Comment) | Organism |
---|---|
crystals of FTHFS complexed with NH4+, K+ or Cs+ are grown by vapor diffusion from 46% saturated ammonium sulfate, 1 mM dithiothreitol and 1% polyehtylene glycol 1000 in 50 mM potassium maleate buffer, pH 7.6, crystals diffract to 3.0-3.2 A resolution | Moorella thermoacetica |
Protein Variants | Comment | Organism |
---|---|---|
E98D | 36% of wild-type activity | Moorella thermoacetica |
E98Q | 93% of wild-type activity, slight increase in thermal stability in the absence of monovalent cation | Moorella thermoacetica |
E98S | 61% of wild-type activity | Moorella thermoacetica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | monovalent cation required for activity | Moorella thermoacetica | |
NH4+ | monovalent cation required for activity | Moorella thermoacetica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
4 * 60000, SDS-PAGE | Moorella thermoacetica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + formate + tetrahydrofolate | Moorella thermoacetica | - |
ADP + phosphate + 10-formyltetrahydrofolate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moorella thermoacetica | P21164 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant FTHFS | Moorella thermoacetica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
780 | - |
recombinant FTHFS | Moorella thermoacetica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + formate + tetrahydrofolate | - |
Moorella thermoacetica | ADP + phosphate + 10-formyltetrahydrofolate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 60000, SDS-PAGE | Moorella thermoacetica |
Synonyms | Comment | Organism |
---|---|---|
FTHFS | - |
Moorella thermoacetica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 60 | - |
Moorella thermoacetica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
69 | - |
in the absence of monovalent cation | Moorella thermoacetica |
79 | - |
in the presence of 200 mM K+ | Moorella thermoacetica |