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Literature summary for 6.3.4.3 extracted from
- Site-directed mutagenesis of a potential catalytic and formyl phosphate binding site and substrate inhibition of N10-formyltetrahydrofolate synthetase (2002), Arch. Biochem. Biophys., 408, 137-143.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Moorella thermoacetica |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R97E | no activity | Moorella thermoacetica |
| R97S | 35% of wild-type kcat | Moorella thermoacetica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.087 | - |
ATP | 27°C, pH 7.0, wild-type FTHFS | Moorella thermoacetica |  |
| 0.16 | - |
ATP | 27°C, pH 7.0, R97S mutant FTHFS | Moorella thermoacetica | |
| 0.46 | - |
formate | 27°C, pH 7.0, wild-type FTHFS | Moorella thermoacetica | |
| 0.95 | - |
formate | 27°C, pH 7.0, R97S mutant FTHFS | Moorella thermoacetica | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + formate + tetrahydrofolate | Moorella thermoacetica | - |
ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Moorella thermoacetica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant FTHFS | Moorella thermoacetica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + carbamoylphosphate + tetrahydrofolate | - |
Moorella thermoacetica | ATP + ? | - |
? | |
| ATP + formate + tetrahydrofolate | - |
Moorella thermoacetica | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FTHFS | - |
Moorella thermoacetica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.39 | - |
ATP | 27°C, pH 7.0, R97S mutant FTHFS | Moorella thermoacetica | |
| 0.4 | - |
formate | 27°C, pH 7.0, R97S mutant FTHFS | Moorella thermoacetica | |
| 0.99 | - |
ATP | 27°C, pH 7.0, wild-type FTHFS | Moorella thermoacetica | |
| 1.13 | - |
formate | 27°C, pH 7.0, wild-type FTHFS | Moorella thermoacetica | |
| 6.08 | - |
ATP | 27°C, pH 7.0, wild-type FTHFS | Moorella thermoacetica | |
| 6.08 | - |
formate | 27°C, pH 7.0, wild-type FTHFS | Moorella thermoacetica | |
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Release 2023.1 (January 2023)
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