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Literature summary for 6.3.4.3 extracted from

  • Nour, J.M.; Rabinowitz, J.C.
    Isolation, characterization, and structural organization of 10-formyltetrahydrofolate synthetase from spinach leaves (1991), J. Biol. Chem., 266, 18363-18369.
    View publication on PubMed
Search for more literature for 6.3.4.3

General Stability

General Stability Organism
enzyme is unstable in phosphate buffers in the absence of high concentrations of salt Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ in presence of Mg2+ Spinacia oleracea
Mn2+ in presence of Mg2+ Spinacia oleracea
Zn2+ in presence of Mg2+ Spinacia oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ Spinacia oleracea
Ca2+ Km for CaCl2: 0.21 mM Spinacia oleracea
Cs+ activity is stimulated by various monovalent cations. The order of effectiveness of 10, 50 or 100 mM salts: Rb+, K+, NH4+, Na+, Cs+ Spinacia oleracea
divalent metal ion the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ Spinacia oleracea
K+ activity is stimulated by various monovalent cations. The order of effectiveness of 10, 50 or 100 mM salts: Rb+, K+, NH4+, Na+, Cs+ Spinacia oleracea
Mg2+ the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ Spinacia oleracea
Mg2+ Km for MgCl2: 0.44 mM Spinacia oleracea
Mn2+ the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ Spinacia oleracea
Mn2+ Km for MnCl2: 0.23 mM Spinacia oleracea
monovalent cations activity is stimulated by various monovalent cations. The order of effectiveness of 10, 50 or 100 mM salts: Rb+, K+,NH4+, Na+,Cs+ Spinacia oleracea
Na+ activity is stimulated by various monovalent cations. The order of effectiveness of 10, 50 or 100 mM salts: Rb+, K+, NH4+, Na+, Cs+ Spinacia oleracea
NH4+ activity is stimulated by various monovalent cations. The order of effectiveness of 10, 50 or 100 mM salts: Rb+, K+, NH4+, Na+, Cs+ Spinacia oleracea
Rb+ activity is stimulated by various monovalent cations. The order of effectiveness of 10, 50 or 100 mM salts: Rb+, K+, NH4+, Na+, Cs+ Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 2 * 67000, SDS-PAGE Spinacia oleracea
130000
-
 gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + formate + tetrahydrofolate Spinacia oleracea enzyme of one-carbon metabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
 spinach
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
202
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + formate + tetrahydrofolate
-
 Spinacia oleracea ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
ATP + formate + tetrahydrofolate enzyme of one-carbon metabolism Spinacia oleracea ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 67000, SDS-PAGE Spinacia oleracea