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Literature summary for 6.3.4.22 extracted from

  • Terasaka, N.; Kimura, S.; Osawa, T.; Numata, T.; Suzuki, T.
    Biogenesis of 2-agmatinylcytidine catalyzed by the dual protein and RNA kinase TiaS (2011), Nat. Struct. Mol. Biol., 18, 1268-1274.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C17A complete loss of activity Archaeoglobus fulgidus
C17S complete loss of activity Archaeoglobus fulgidus
C210S complete loss of activity Archaeoglobus fulgidus
D11A complete loss of activity Archaeoglobus fulgidus
D8A complete loss of activity Archaeoglobus fulgidus
D9A complete loss of activity Archaeoglobus fulgidus
E159A complete loss of activity Archaeoglobus fulgidus
F286A complete loss of activity Archaeoglobus fulgidus
F301A complete loss of activity Archaeoglobus fulgidus
I143A reduced activity Archaeoglobus fulgidus
I49A reduced activity Archaeoglobus fulgidus
K52A complete loss of activity Archaeoglobus fulgidus
N194A reduced activity Archaeoglobus fulgidus
N56A complete loss of activity Archaeoglobus fulgidus
R217A complete loss of activity Archaeoglobus fulgidus
R369A complete loss of activity Archaeoglobus fulgidus
R44A complete loss of activity Archaeoglobus fulgidus
R54A complete loss of activity Archaeoglobus fulgidus
T18A complete loss of activity Archaeoglobus fulgidus
T18D complete loss of activity Archaeoglobus fulgidus
T53A complete loss of activity Archaeoglobus fulgidus

Inhibitors

Inhibitors Comment Organism Structure
alpha,beta-methyleneadenosine 5'-triphosphate
-
Archaeoglobus fulgidus
beta,gamma-methyleneadenosine 5'-triphosphate
-
Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00029
-
[tRNAIle2]-cytidine34 pH 8.8, 70°C Archaeoglobus fulgidus
0.00087
-
agmatine pH 8.8, 70°C Archaeoglobus fulgidus
0.006
-
ATP pH 8.8, 70°C Archaeoglobus fulgidus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl optimal concentration: 100 mM Archaeoglobus fulgidus
Mg2+ optimal concentration: 2-5 mM Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + agmatine + [tRNAIle2]-cytidine34 Archaeoglobus fulgidus the enzyme catalyzes the essential modification at the anticodon wobble position (position 34) of the AUA-codon of tRNAIle [tRNAIle2]-2-agmatinylcytidine34 + AMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O28025
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the Thr18-Cyt34 kinase domain autophosphorylates the Thr18 of the enzyme Archaeoglobus fulgidus

Purification (Commentary)

Purification (Comment) Organism
-
Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + agmatine + [tRNAIle2]-cytidine34 the enzyme catalyzes the essential modification at the anticodon wobble position (position 34) of the AUA-codon of tRNAIle Archaeoglobus fulgidus [tRNAIle2]-2-agmatinylcytidine34 + AMP + diphosphate
-
?
ATP + agmatine + [tRNAIle2]-cytidine34 the precursor form of tRNAIle bearing an unmodified cytidine at the wobble position (C34) is aminoacylated with methionine (Met) and decodes the AUG codon.The Thr18-Cyt34 kinase domain first hydrolyzes ATP into AMP and diphosphate, then phosphorylates the C2 position of cytidine34 with the gamma-phosphate. Next, the amino group of agmatine attacks this position to release the phosphate and form 2-agmatinylcytidine. The Thr18-Cyt34 kinase domain also autophosphorylates the Thr18 of the enzyme, which may be involved in 2-agmatinylcytidine formation. Catalytic efficiency (kcat/Km) with GTP, UTP or CTP as substrate is less than 1% compared to ATP Archaeoglobus fulgidus [tRNAIle2]-2-agmatinylcytidine34 + AMP + diphosphate
-
?

Synonyms

Synonyms Comment Organism
TiaS
-
Archaeoglobus fulgidus
tRNAIle-agm2C synthetase
-
Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Archaeoglobus fulgidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.006
-
agmatine pH 8.8, 70°C Archaeoglobus fulgidus
0.0062
-
ATP pH 8.8, 70°C Archaeoglobus fulgidus
0.0065
-
[tRNAIle2]-cytidine34 pH 8.8, 70°C Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
assay at Archaeoglobus fulgidus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00037
-
pH 8.8, 70°C Archaeoglobus fulgidus alpha,beta-methyleneadenosine 5'-triphosphate
0.00079
-
pH 8.8, 70°C Archaeoglobus fulgidus beta,gamma-methyleneadenosine 5'-triphosphate

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
ATP pH 8.8, 70°C Archaeoglobus fulgidus
6.9
-
agmatine pH 8.8, 70°C Archaeoglobus fulgidus
22.41
-
[tRNAIle2]-cytidine34 pH 8.8, 70°C Archaeoglobus fulgidus