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Literature summary for 6.3.4.22 extracted from

  • Ikeuchi, Y.; Kimura, S.; Numata, T.; Nakamura, D.; Yokogawa, T.; Ogata, T.; Wada, T.; Suzuki, T.; Suzuki, T.
    Agmatine-conjugated cytidine in a tRNA anticodon is essential for AUA decoding in archaea (2010), Nat. Chem. Biol., 6, 277-282.
    View publication on PubMed
Search for more literature for 6.3.4.22

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Archaeoglobus fulgidus

Protein Variants

Protein Variants Comment Organism
C352A/C355A almost no 2-agmatinylcytidine formation is observed Archaeoglobus fulgidus
G218A mutation reduces activity Archaeoglobus fulgidus
R140/G141A mutation completely abolishes activity Archaeoglobus fulgidus
R164A mutation reduces activity Archaeoglobus fulgidus
R217A mutation reduces activity Archaeoglobus fulgidus
T248A/D249A mutation reduces activity Archaeoglobus fulgidus
Y163A mutation reduces activity Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + agmatine + [tRNAIle2]-cytidine34 Archaeoglobus fulgidus
-
 [tRNAIle2]-2-agmatinylcytidine34 + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O28025
-
-
Pyrococcus horikoshii O59476
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + agmatine + [tRNAIle2]-cytidine34
-
 Archaeoglobus fulgidus [tRNAIle2]-2-agmatinylcytidine34 + ?
-
 ?
ATP + agmatine + [tRNAIle2]-cytidine34
-
 Pyrococcus horikoshii [tRNAIle2]-2-agmatinylcytidine34 + ?
-
 ?

Synonyms

Synonyms Comment Organism
AF2259
-
 Archaeoglobus fulgidus
AF2259
-
 Pyrococcus horikoshii
TiaS
-
 Archaeoglobus fulgidus
TiaS
-
 Pyrococcus horikoshii
tRNAIle-2-agmatinylcytidine synthetase
-
 Archaeoglobus fulgidus
tRNAIle-2-agmatinylcytidine synthetase
-
 Pyrococcus horikoshii
tRNAIle-agm2C synthetase
-
 Archaeoglobus fulgidus
tRNAIle-agm2C synthetase
-
 Pyrococcus horikoshii
tRNAIle-agmatidine synthetase
-
 Archaeoglobus fulgidus
tRNAIle-agmatidine synthetase
-
 Pyrococcus horikoshii

General Information

General Information Comment Organism
physiological function modification at the anticodon wobble position of archaeal tRNAIle2. According to the chemical structure of agm2C, conjugation of an agmatine moiety at the C2 position of cytosine induces a tautomeric conversion with protonation of the N3 position and imino group formation at the C4 position. This modification completely alters the proton donor-acceptor pattern of cytosine in Watson-Click hydrogen bonding to that of uracil, enabling agm2C to base pair with adenine instead of guanine. tRNAIle2 acquires the ability to decode the AUA codon via formation 2-agmatinylcytidine Archaeoglobus fulgidus
physiological function modification at the anticodon wobble position of archaeal tRNAIle2. According to the chemical structure of agm2C, conjugation of an agmatine moiety at the C2 position of cytosine induces a tautomeric conversion with protonation of the N3 position and imino group formation at the C4 position. This modification completely alters the proton donor-acceptor pattern of cytosine in Watson-Click hydrogen bonding to that of uracil, enabling agm2C to base pair with adenine instead of guanine. tRNAIle2 acquires the ability to decode the AUA codon via formation 2-agmatinylcytidine Pyrococcus horikoshii