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Literature summary for 6.3.4.20 extracted from

  • Cicmil, N.; Huang, R.
    Crystal structure of QueC from Bacillus subtilis: an enzyme involved in preQ1 biosynthesis (2008), Proteins, 72, 1084-1088.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3) Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
purified QueC, vapor diffusion method, 10 mg/ml protein is mixed with crystallization solution containing 25% PEG 6000, 100 mM NaCl, 10 mM 1,6 hexanediol, and 100 mM MES, pH 5.5, 1 week, X-ray diffraction structure determination and analysis at 2.95 A resolution Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bacillus subtilis
Zn2+ coordination of a zinc ion by the conserved C(x)8CxxCxxC motif in QueC, binding structure, overview Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O31675
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant QueC from Escherichia coli strain BL21(DE3) by anion exchange chromatography, hydrophobic interaction chromatography, and another different step of anion exchange chromatography, followed by gel filtration Bacillus subtilis

Subunits

Subunits Comment Organism
dimer or tetramer the biologically relevant unit is likely to be a QueC homodimer, crystal structure. Monomer structure, overview. The Rossmann fold of the N-terminal part of QueC, in combination with the C-terminal zinc-binding motif, forms a cavity in QueC of substantial size, which is likely to be the location of substrate binding Bacillus subtilis

Synonyms

Synonyms Comment Organism
queC
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Bacillus subtilis

General Information

General Information Comment Organism
metabolism the products of four genes, queC, queD, queE, and queF, are involved in preQ1 biosynthesis with GTP as the starting material. preQ1 is transformed to Q in tRNA Bacillus subtilis