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Literature summary for 6.3.4.2 extracted from
- Activation and inhibition of CTP synthase from Trypanosoma brucei, the causative agent of African sleeping sickness (2011), Bioorg. Med. Chem. Lett., 21, 5188-5190.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| GTP | activates glutamine-dependent CTP formation at concentrations below 0.2 mM | Trypanosoma brucei |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Trypanosoma brucei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Caffeine | - |
Trypanosoma brucei | |
| GTP | inhibits glutamine-dependent CTP formation at concentrations above 0.2 mM | Trypanosoma brucei | |
| guanosine | - |
Trypanosoma brucei | |
| uric acid | - |
Trypanosoma brucei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
x * 70000, SDS-PAGE | Trypanosoma brucei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + UTP + L-glutamine | Trypanosoma brucei | - |
ADP + phosphate + CTP + L-glutamate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-IDA resin column chromatography | Trypanosoma brucei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + UTP + L-glutamine | - |
Trypanosoma brucei | ADP + phosphate + CTP + L-glutamate | - |
ir | |
| additional information | the enzyme can utilize NH3 as a substrate | Trypanosoma brucei | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70000, SDS-PAGE | Trypanosoma brucei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CTP synthase | - |
Trypanosoma brucei |
| CTPS | - |
Trypanosoma brucei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Trypanosoma brucei | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
in HEPES buffer (70 mM, pH 7.3), at 37°C | Trypanosoma brucei | uric acid | |
| 0.38 | - |
in HEPES buffer (70 mM, pH 7.3), at 37°C | Trypanosoma brucei | guanosine | |
| 0.46 | - |
in HEPES buffer (70 mM, pH 7.3), at 37°C | Trypanosoma brucei | GTP | |
| 0.48 | - |
in HEPES buffer (70 mM, pH 7.3), at 37°C | Trypanosoma brucei | Caffeine | |
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