Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the C-terminal regulatory domain, residues Ser562-Asp591, of CTPS1 greatly increases the Vmax of the enzyme | Homo sapiens |
S568A | 2fold increase in Km value for UTP. Mutation of S568A significantly increases CTPS2 activity. The S568A mutation has a greater effect on the glutamine than ammonia-dependent activity | Homo sapiens |
S571A | 4fold increase in Km value for UTP | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CTP | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | maximally active at physiological concentrations of ATP, GTP, and glutamine, whereas the Km and IC50 values for the substrate UTP and the product CTP, respectively, are close to their physiological concentrations | Homo sapiens | |
0.027 | - |
L-Gln | wild-type, pH 8.1, 37°C | Homo sapiens | |
0.072 | - |
L-Gln | mutant S568A, pH 8.1, 37°C | Homo sapiens | |
0.1 | - |
L-Gln | wild-type, pH 8.1, 37°C | Homo sapiens | |
0.19 | - |
UTP | wild-type, pH 8.1, 37°C | Homo sapiens | |
0.42 | - |
UTP | mutant S568A, pH 8.1, 37°C | Homo sapiens | |
0.59 | - |
UTP | wild-type, pH 8.1, 37°C | Homo sapiens | |
0.8 | - |
UTP | mutant S571A, pH 8.1, 37°C | Homo sapiens | |
1.9 | - |
UTP | C-terminal deletion mutant, pH 8.1, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | Q9NRF8 | CTP synthase 2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | Low serum treatment increases CTPS2 phosphorylation. Ser568 and Ser571 are two major phosphorylation sites, and Ser568 is phosphorylated by casein kinase 1 both in vitro and in vivo. Mutation of S568A but not S571A significantly increases CTPS2 activity. The S568A mutation has a greater effect on the glutamine than ammonia-dependent activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UTP + L-Gln | - |
Homo sapiens | ADP + phosphate + CTP + L-Glu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CTPS1 | - |
Homo sapiens |
CTPS2 | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.04 | - |
wild-type, pH 8.1, 37°C | Homo sapiens | CTP | |
0.042 | - |
wild-type, pH 8.1, 37°C | Homo sapiens | CTP | |
0.045 | - |
mutant S571A, pH 8.1, 37°C | Homo sapiens | CTP | |
0.057 | - |
C-terminal deletion mutant, pH 8.1, 37°C | Homo sapiens | CTP | |
0.072 | - |
mutant S568A, pH 8.1, 37°C | Homo sapiens | CTP |