Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | required, activates | Escherichia coli | |
additional information | binding of the substrates ATP and UTP, or the product CTP, promotes oligomerization of CTPS from inactive dimers to active tetramers, Gly142 is critical for nucleotide-dependent oligomerization of CTPS to active tetramers | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
G142A | site-directed mutagenesis, inactive mutant with both ammonia and glutamine | Escherichia coli |
G143A | site-directed mutagenesis, kcat/Km for ammonia-dependent and glutamine-dependent CTP formation by mutant G143A are reduced by 22fold and 16fold, respectively, compared to the wild-type enzyme. The mutant is able to form active tetramers in the presence of ATP and UTP | Escherichia coli |
G146A | site-directed mutagenesis, kcat/Km for ammonia-dependent and glutamine-dependent CTP formation by mutant G143A are reduced by 1.4fold and 1.8fold, respectively, compared to the wild-type enzyme. The mutant is able to form active tetramers in the presence of ATP and UTP | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of wild-type and mutants | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UTP + L-glutamine | Escherichia coli | - |
ADP + phosphate + CTP + L-glutamate | - |
? | |
ATP + UTP + NH3 | Escherichia coli | - |
ADP + phosphate + CTP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A7E5 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UTP + L-glutamine | - |
Escherichia coli | ADP + phosphate + CTP + L-glutamate | - |
? | |
ATP + UTP + L-glutamine | CTPS catalyses the ATP-dependent formation of CTP from UTP using either ammonia or L-glutamine as the nitrogen source | Escherichia coli | ADP + phosphate + CTP + L-glutamate | - |
? | |
ATP + UTP + NH3 | - |
Escherichia coli | ADP + phosphate + CTP | - |
? | |
ATP + UTP + NH3 | CTPS catalyses the ATP-dependent formation of CTP from UTP using either ammonia or L-glutamine as the nitrogen source | Escherichia coli | ADP + phosphate + CTP | - |
? | |
additional information | UTP- and CTP-binding structures, overview | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | binding of the substrates ATP and UTP, or the product CTP, promotes oligomerization of CTPS from inactive dimers to active tetramers, Gly142 is critical for nucleotide-dependent oligomerization of CTPS to active tetramers. Oligomerization of Gly mutant enzymes, overview | Escherichia coli |
tetramer | conserved Gly142 is critical for tetramerization, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
CTP synthase | - |
Escherichia coli |
CTPS | - |
Escherichia coli |
cytidine 5'-triphosphate synthase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
UTP | pH 8.0, 37°C, mutant G142A with L-glutamine, in presence of 0.25 mM GTP | Escherichia coli | |
0.67 | - |
UTP | pH 8.0, 37°C, mutant G143A with L-glutamine, in presence of 0.25 mM GTP | Escherichia coli | |
4.2 | - |
UTP | pH 8.0, 37°C, mutant G146A with L-glutamine, in presence of 0.25 mM GTP | Escherichia coli | |
5 | - |
UTP | pH 8.0, 37°C, wild-type enzyme with L-glutamine, in presence of 0.25 mM GTP | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Escherichia coli |