Application | Comment | Organism |
---|---|---|
drug development | structure serves as a basis for structure-based design of anti-proliferative inhibitors | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
cDNA encoding the synthetase domain of human CTPS (isoform 1) is subcloned into the pNIC-Bsa4 vector, the resulting construct codes for a protein with an N-terminal hexahistidine tag with an integrated TEV protease-cleavage site, the entire plasmid is transformed in Escherichia coli BL-21 | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystal structure analysis discloses a homotetrameric structure and each active site is formed by three different subunits. Sulfate ions bound to the active sites indicate the positions of phosphate-binding sites for the substrates ATP and UTP and the feedback inhibitor CTP. Together with earlier structures of bacterial CTPS, the human CTPS structure provides an extended understanding of the structurefunction relationship of CTPS-family members | Homo sapiens |
the protein is crystallized by the hanging-drop method, equilibrating 1 ml protein solution and 1 ml of a solution containing 0.1 M Tris pH 8.8, 1.2 M (NH4)2SO4 and 50 mM malonic acid against 0.5 ml of a well solution containing 0.1 M Tris pH 8.8 and 1.2 M (NH4)2SO4. Crystals with a maximum dimension of 0.03 nm formed in 3 d | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
protein is purified by using HisTrap HP and Superdex 75 columns | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
CTS | - |
Homo sapiens |
cytidine triphosphate synthetase | - |
Homo sapiens |