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Literature summary for 6.3.4.2 extracted from

  • Lunn, F.A.; Bearne, S.L.
    Alternative substrates for wild-type and L109A E. coli CTP synthases: kinetic evidence for a constricted ammonia tunnel (2004), Eur. J. Biochem., 271, 4204-4212.
    View publication on PubMed
Search for more literature for 6.3.4.2

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and L109A recombinant enzyme are expressed in Escherichia coli BL21(DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
L109A uncoupling of the hydrolysis of gamma-glutamyl hydroxamate and nascent NH2OH production from N4-hydroxy-CTP formation is more pronounced with mutant than with wild-type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
 Gln-OH 37°C, pH 8.0, mutant enzyme L109A Escherichia coli
0.165
-
 Gln-OH 37°C, pH 8.0, wild-type enzyme Escherichia coli
0.354
-
 Gln 37°C, pH 8.0, wild-type enzyme Escherichia coli
0.497
-
 Gln 37°C, pH 8.0, mutant enzyme L109A Escherichia coli
2.15
-
 NH3 37°C, pH 8.0, wild-type enzyme Escherichia coli
2.17
-
 NH3 37°C, pH 8.0, mutant enzyme L109A Escherichia coli
39.4
-
 L-glutamine 37°C, pH 8.0, wild-type enzyme Escherichia coli
75.3
-
 NH2OH 37°C, pH 8.0, mutant enzyme L109A Escherichia coli
82.8
-
 NH2OH 37°C, pH 8.0, wild-type enzyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A7E5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzyme L109A Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UTP + gamma-L-glutamylhydroxamate
-
 Escherichia coli ADP + phosphate + N4-hydroxy-CTP
-
 ?
ATP + UTP + Gln
-
 Escherichia coli ADP + phosphate + CTP + Glu
-
 ?
ATP + UTP + L-gamma-glutamyl hydrazide
-
 Escherichia coli ADP + phosphate + N4-amino-CTP + L-glutamate
-
 ?
ATP + UTP + NH2NH2
-
 Escherichia coli ADP + phosphate + CTP + N4-amino CTP
-
 ?
ATP + UTP + NH2OH
-
 Escherichia coli ADP + phosphate + N4-hydroxy-CTP
-
 ?
ATP + UTP + NH4+
-
 Escherichia coli ADP + phosphate + CTP
-
 ?
L-glutamine + H2O
-
 Escherichia coli L-glutamate + NH3
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.063
-
 Gln-OH 37°C, pH 8.0, mutant enzyme L109A Escherichia coli
0.453
-
 Gln-OH 37°C, pH 8.0, wild-type enzyme Escherichia coli
1.41
-
 L-glutamine 37°C, pH 8.0, wild-type enzyme Escherichia coli
1.86
-
 Gln 37°C, pH 8.0, mutant enzyme L109A Escherichia coli
6.1
-
 Gln 37°C, pH 8.0, wild-type enzyme Escherichia coli
9.5
-
 NH3 37°C, pH 8.0, wild-type enzyme Escherichia coli
10.1
-
 NH3 37°C, pH 8.0, mutant enzyme L109A Escherichia coli
14
-
 NH2OH 37°C, pH 8.0, wild-type enzyme Escherichia coli
14.1
-
 NH2OH 37°C, pH 8.0, mutant enzyme L109A Escherichia coli