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Literature summary for 6.3.4.2 extracted from
- Phosphorylation and regulation of CTP synthetase from Saccharomyces cerevisiae by protein kinase A (1996), J. Biol. Chem., 271, 28777-28783.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CTP | IC50 for the native enzyme: 0.12 in presence of 0.5 mM ATP, 0.22 mM in presence of 1 mM ATP. IC50 for the phosphorylated enzyme: 0.21 mM in presence of 0.5 mM ATP, 0.31 mM in presence of 1 mM ATP | Saccharomyces cerevisiae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
UTP | pH 8.0, 30°C, phosphorylated enzyme, in presence of 2 mM ATP | Saccharomyces cerevisiae | |
| 0.05 | - |
UTP | pH 8.0, 30°C, native enzyme, in presence of 2 mM ATP | Saccharomyces cerevisiae | |
| 0.08 | - |
UTP | pH 8.0, 30°C, native enzyme or phosphorylated enzyme, in presence of 0.5 mM ATP | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | phosphorylation of CTP synthetase by protein kinase A results in the stimulation of CTP synthetase activity. The mechanism of stimulation involves an increase in Vmax of the reaction and an increase of the enzyme affinity for ATP | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + UTP + glutamine | - |
Saccharomyces cerevisiae | ADP + phosphate + CTP + Glu | - |
? | |
| ATP + UTP + NH4+ | - |
Saccharomyces cerevisiae | ADP + phosphate + CTP | - |
? | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
IC50 for the native enzyme: 0.12 in presence of 0.5 mM ATP, 0.22 mM in presence of 1 mM ATP. IC50 for the phosphorylated enzyme: 0.21 mM in presence of 0.5 mM ATP, 0.31 mM in presence of 1 mM ATP | Saccharomyces cerevisiae | CTP | |
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