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Literature summary for 6.3.4.2 extracted from
- Cytidine triphosphate synthetase (1978), Methods Enzymol., 51, 79-83.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
4 * 52000, enzyme exists as tetramer in presence of UTP, Mg2+ and ATP | Escherichia coli |
| 105000 | - |
dimer, gel filtration | Escherichia coli |
| 210000 | - |
tetramer | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.8 | 6.1 | - |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, enzyme concentration 1-4 mg/ml, 20 mM sodium phosphate buffer, pH 7.2, 2 mM L-glutamine, 1 mM EDTA, 70 mM 2-mercaptoethanol, 20% glycerol, stable for 3 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + UTP + NH4+ | - |
Escherichia coli | ADP + phosphate + CTP | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 52000, enzyme exists as tetramer in presence of UTP, Mg2+ and ATP | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Escherichia coli |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | - |
- |
5.5 |
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Release 2023.1 (January 2023)
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