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Literature summary for 6.3.4.2 extracted from

  • Wadskov-Hansen, S.L.; Willemoes, M.; Martinussen, J.; Hammer, K.; Neuhard, J.; Larsen, S.
    Cloning and verification of the Lactococcus lactis pyrG gene and characterization of the gene product, CTP synthase (2001), J. Biol. Chem., 276, 38002-38009.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Lactococcus lactis

General Stability

General Stability Organism
the tetramer is very stable even at dilute enzyme concentrations Lactococcus lactis

Inhibitors

Inhibitors Comment Organism Structure
CTP cooperative inhibition Lactococcus lactis
dCTP very weak inhibitor Lactococcus lactis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, the activation curve for free Mg2+ concentration is greatly dependent on whether the concentration of ATP and UTP are near saturating Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
2 * 60000 Lactococcus lactis
234000
-
gel filtration Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Lactococcus lactis in Lactococcus lactis the pyrG gene product is the only enzyme responsible for the amination of UTP to CTP ?
-
?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
subsp. cremonis
-

Storage Stability

Storage Stability Organism
-20°C, stable for several months Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dUTP + NH4+ half-saturating concentration of 0.6 mM for dUTP, it is unlikely that this reaction plays a significant physiological role Lactococcus lactis ADP + phosphate + dCTP
-
?
ATP + UTP + NH4+
-
Lactococcus lactis ADP + phosphate + CTP
-
?
additional information in Lactococcus lactis the pyrG gene product is the only enzyme responsible for the amination of UTP to CTP Lactococcus lactis ?
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 60000 Lactococcus lactis