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Literature summary for 6.3.4.2 extracted from

  • Ostrander, D.B.; O'Brien, D.J.; Gorman, J.A.; Carman, G.M.
    Effect of CTP synthetase regulation by CTP on phospholipid synthesis in Saccharomyces cerevisiae (1998), J. Biol. Chem., 273, 18992-19001.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
E161K specific activity of the mutant URA7-encoded and URA8-encoded enzymes are 2fold greater when compared with the wild-type enzymes. The mutant enzymes are less sensitive to CTP product inhibition with inhibitor constants for CTP of 8.4fold- and 5.5fold greater, respectively, than those of their wild-type counterparts. Cells expressing the E161K mutant enzymes on a multicopy plasmid exhibit an increase in resistance to the pyrimidine poison and cancer therapeutic drug cyclopentenylcytosine and accumulate elevated levels of CTP when compared with cells expressing the wild-type enzymes. Cells expressing the E161K mutation in the URA7-encoded CTP synthetase exhibit an 1.5fold increase in the utilization of the Kennedy pathway for phosphatidylcholine synthesis when compared with control cells. Cells bearing the mutation also exhibit an 1.5fold increase in the synthesis of phosphatidylcholine, 1.3fold for phosphatidylethanolamine and 2fold for phosphatidate and a 1.7fold decrease in synthesis of phosphatidylserine. Cells bearing the e161K mutation exhibit an 1.6fold increase in the ratio of total neutral lipids to phospholipids, an 1.4fold increase in triacylglycerol, a 1.7fold increase in free fatty acids, an1.8fold increase in ergosterol ester and a 1.3fold decrease in diacylgylcerol when compared with control cells Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
CTP product inhibition of wild-type enzyme, mutant E161K is less sensitive to CTP product inhibition Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae regulation of CTP synthetase activity by CTP plays an important role in the regulation of phospholipid synthesis ?
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Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UTP + NH4+
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 Saccharomyces cerevisiae ADP + phosphate + CTP
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additional information regulation of CTP synthetase activity by CTP plays an important role in the regulation of phospholipid synthesis Saccharomyces cerevisiae ?
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