Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | required as an allosteric effector to promote glutamine hydrolysis | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D107A | enzyme exhibits wild-type NH3-dependent activity and affinity for glutamine, but impaired glutamine-dependent CTP formation, affinity of the mutant enzyme for GTP is reduced 2-4fold | Escherichia coli |
E103A | mutant enzyme exhibits no glutamine-dependent activity and is only partially active with NH3 | Escherichia coli |
G110A | affinity of the mutant enzyme for GTP is reduced 2-4fold, enzyme exhibits wild-type NH3-dependent activity and affinity for glutamine, but impaired glutamine-dependent CTP formation | Escherichia coli |
H118A | mutant enzyme exhibits no glutamine-dependent activity and is only partially active with NH3 | Escherichia coli |
K102A | mutant enzyme exhibits wild-type activity with respect to NH3 and glutamine | Escherichia coli |
L109A | enzyme exhibits wild-type NH3-dependent activity and affinity for glutamine, but impaired glutamine-dependent CTP formation, affinity of the mutant enzyme for GTP is reduced 2-4fold | Escherichia coli |
R104A | mutant enzyme exhibits no glutamine-dependent activity and is only partially active with NH3 | Escherichia coli |
R105A | enzyme exhibits wild-type NH3-dependent activity and affinity for glutamine, but impaired glutamine-dependent CTP formation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UTP + glutamine | - |
Escherichia coli | ADP + phosphate + CTP + Glu | - |
? | |
ATP + UTP + NH4+ | - |
Escherichia coli | ADP + phosphate + CTP | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
NH3 | pH 8.0, mutant enzyme H118A | Escherichia coli | |
0.92 | - |
NH3 | pH 8.0, mutant enzyme E103A | Escherichia coli | |
1.03 | - |
Gln | pH 8.0, mutant enzyme L109F | Escherichia coli | |
1.53 | - |
Gln | pH 8.0, mutant enzyme R105A | Escherichia coli | |
1.64 | - |
Gln | pH 8.0, mutant enzyme L109A | Escherichia coli | |
1.96 | - |
NH3 | pH 8.0, mutant enzymeR104A | Escherichia coli | |
2.17 | - |
Gln | pH 8.0, mutant enzyme G110A | Escherichia coli | |
3.5 | - |
Gln | pH 8.0, mutant enzyme D107A | Escherichia coli | |
4.18 | - |
NH3 | pH 8.0, mutant enzyme G110A | Escherichia coli | |
4.22 | - |
Gln | pH 8.0, mutant enzyme K102A | Escherichia coli | |
6.1 | - |
Gln | pH 8.0, wild-type enzyme | Escherichia coli | |
7.97 | - |
NH3 | pH 8.0, mutant enzyme L109A | Escherichia coli | |
8.7 | - |
NH3 | pH 8.0, mutant enzyme D107A | Escherichia coli | |
8.88 | - |
NH3 | pH 8.0, mutant enzyme R105A | Escherichia coli | |
9.4 | - |
NH3 | pH 8.0, wild-type enzyme | Escherichia coli | |
11 | - |
NH3 | pH 8.0, mutant enzyme L109F | Escherichia coli | |
12.2 | - |
NH3 | pH 8.0, mutant enzyme K102A | Escherichia coli |