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Literature summary for 6.3.4.19 extracted from

  • Ikeuchi, Y.; Soma, A.; Ote, T.; Kato, J.; Sekine, Y.; Suzuki, T.
    Molecular mechanism of lysidine synthesis that determines tRNA identity and codon recognition (2005), Mol. Cell, 19, 235-246.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information a mutation study reveals that Escherichia coli TilS discriminates tRNAIle from the structurally similar tRNAMet having the same anticodon loop by recognizing the anticodon loop, the anticodon stem, and the acceptor stem Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic parameters of Escherichia coli TilS with various tRNAIle2 mutants and with tRNAMet mutants Escherichia coli
0.00038
-
[tRNAIle2]-cytidine34 unmodified tRNAIle2, pH 7.8, 37°C Escherichia coli
0.22
-
ATP pH 7.8, 37°C Escherichia coli
0.38
-
L-lysine pH 7.8, 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[tRNAIle2]-cytidine34 + L-lysine + ATP Escherichia coli
-
[tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[tRNAIle2]-cytidine34 + L-lysine + ATP
-
Escherichia coli [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate
-
?
[tRNAIle2]-cytidine34 + L-lysine + ATP lysidine synthesis consists of two consecutive reactions that involve an adenylated tRNA intermediate. A mutation study reveals that Escherichia coli TilS discriminates tRNAIle from the structurally similar tRNAMet having the same anticodon loop by recognizing the anticodon loop, the anticodon stem, and the acceptor stem. TilS binds to the anticodon region and 3' side of the acceptor stem, which cover the recognition sites. A dedicated mechanism is embedded in tRNAIle that controls its recognition and discrimination by TilS Escherichia coli [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate
-
?

Synonyms

Synonyms Comment Organism
TilS
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information kinetic parameters of Escherichia coli TilS with various tRNAIle2 mutants and with tRNAMet mutants Escherichia coli
0.012
-
[tRNAIle2]-cytidine34 unmodified tRNAIle2, pH 7.8, 37°C Escherichia coli
0.015
-
ATP pH 7.8, 37°C Escherichia coli
0.07
-
L-lysine pH 7.8, 37°C Escherichia coli

General Information

General Information Comment Organism
physiological function 2-lysyl cytidine is a lysine-containing cytidine derivative commonly found at the wobble position of bacterial AUA codon-specific tRNAIle. This modification determines both codon and amino acid specificities of tRNAIle Escherichia coli