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Literature summary for 6.3.4.18 extracted from

  • Brugarolas, P.; Duguid, E.M.; Zhang, W.; Poor, C.B.; He, C.
    Structural and biochemical characterization of N5-carboxyaminoimidazole ribonucleotide synthetase and N5-carboxyaminoimidazole ribonucleotide mutase from Staphylococcus aureus (2011), Acta Crystallogr. Sect. D, 67, 707-715.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.4.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (DE3) cells Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M bis-Tris pH 6.5, 28% (w/v) polyethylene glycol monomethyl ether 2000 Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
F78I the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme Staphylococcus aureus
F78W the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0139
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole wild type enzyme, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication Staphylococcus aureus
0.0432
-
 ATP wild type enzyme, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication Staphylococcus aureus
0.0513
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole mutant enzyme F78I, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication Staphylococcus aureus
0.103
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole mutant enzyme F78W, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication Staphylococcus aureus
18.8
-
 HCO3- wild type enzyme, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- + H+ Staphylococcus aureus
-
 ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole
-
 ?
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- + H+ Staphylococcus aureus Newman
-
 ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus A0A0H3KFM7
-
-
Staphylococcus aureus Newman A0A0H3KFM7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- + H+
-
 Staphylococcus aureus ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole
-
 ?
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- + H+
-
 Staphylococcus aureus Newman ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole
-
 ?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Staphylococcus aureus

Synonyms

Synonyms Comment Organism
N5-CAIR synthetase
-
 Staphylococcus aureus
N5-carboxyaminoimidazole ribonucleotide synthetase
-
 Staphylococcus aureus
PurK
-
 Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.83
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole mutant enzyme F78I, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication Staphylococcus aureus
18
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole mutant enzyme F78W, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication Staphylococcus aureus
24.83
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole wild type enzyme, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Staphylococcus aureus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
175
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole mutant enzyme F78W, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication Staphylococcus aureus
308
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole mutant enzyme F78I, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication Staphylococcus aureus
1783
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole wild type enzyme, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication Staphylococcus aureus