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Literature summary for 6.3.4.17 extracted from

  • Drake, J.C.; Baram, J.; Allegra, C.J.
    Isolation and characterization of a novel dihydrofolate formylating enzyme from human MCF-7 breast cancer cells (1990), Biochem. Pharmacol., 39, 615-618.
    View publication on PubMed
Search for more literature for 6.3.4.17

General Stability

General Stability Organism
Perchloric acid, 5%, denatures Homo sapiens
Perchloric acid, 5%, denatures Lacticaseibacillus casei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.097
-
 dihydrofolate
-
 Homo sapiens
0.911
-
 dihydrofolate
-
 Lacticaseibacillus casei

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ enhances activity Homo sapiens
K+ enhances activity Lacticaseibacillus casei
Mg2+ required Homo sapiens
Mg2+ required Lacticaseibacillus casei

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Lacticaseibacillus casei
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
MCF-7 cell MCF-7 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + formate + dihydrofolate
-
 Homo sapiens ADP + phosphate + 10-formyldihydrofolate
-
 ?
ATP + formate + dihydrofolate
-
 Lacticaseibacillus casei ADP + phosphate + 10-formyldihydrofolate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 60 s, denaturation Homo sapiens
100
-
 60 s, denaturation Lacticaseibacillus casei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Homo sapiens
8.5
-
-
 Lacticaseibacillus casei