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Literature summary for 6.3.4.16 extracted from

  • Purcarea, C.; Simon, V.; Prieur, D.; Herve, G.
    Purification and characterization of carbamoyl-phosphate synthetase from the deep-sea hyperthermophilic archaebacterium Pyrococcus abyssi (1996), Eur. J. Biochem., 236, 189-199.
    View publication on PubMed
Search for more literature for 6.3.4.16

General Stability

General Stability Organism
stabilty under pressure, progressive inactivation down to 50% of initial activity at 200 MPa, this residual activity is constant at 200 MPa for at least 50 min Pyrococcus abyssi

Inhibitors

Inhibitors Comment Organism Structure
AMP 1 mM, inhibition is more pronounced at 37°C compared to 70°C Pyrococcus abyssi
CTP 0.3 mM, about 50% inhibition, inhibition is more pronounced at 37°C compared to 70°C Pyrococcus abyssi
dCTP 1 mM, inhibition is more pronounced at 37°C compared to 70°C Pyrococcus abyssi
dGTP 1 mM, inhibition is more pronounced at 37°C compared to 70°C Pyrococcus abyssi
ITP 0.3 mM, about 50% inhibition, inhibition is more pronounced at 37°C compared to 70°C Pyrococcus abyssi
TTP 0.3 mM, about 50% inhibition, inhibition is more pronounced at 37°C compared to 70°C Pyrococcus abyssi
UTP 0.3 mM, about 50% inhibition, inhibition is more pronounced at 37°C compared to 70°C Pyrococcus abyssi
XTP 0.3 mM, about 50% inhibition, inhibition is more pronounced at 37°C compared to 70°C Pyrococcus abyssi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3
-
 CO2 NaHCO3, 37°C, pH 8 Pyrococcus abyssi
7.2
-
 CO2 NaHCO3, 70°C, pH 7.5 Pyrococcus abyssi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46600
-
 1 * 46600, SDS-PAGE Pyrococcus abyssi
50700
-
 gel filtration Pyrococcus abyssi

Organism

Organism UniProt Comment Textmining
Pyrococcus abyssi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus abyssi

Storage Stability

Storage Stability Organism
4°C, 6 months, stable Pyrococcus abyssi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NH3 + CO2 + H2O
-
 Pyrococcus abyssi ADP + phosphate + carbamoylphosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 46600, SDS-PAGE Pyrococcus abyssi

Synonyms

Synonyms Comment Organism
carbamoyl-phosphate synthetase
-
 Pyrococcus abyssi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 3 h, 50% loss of activity Pyrococcus abyssi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 70°C Pyrococcus abyssi
7.6 8.6 at 37°C Pyrococcus abyssi

pH Range

pH Minimum pH Maximum Comment Organism
5.7 8.5 pH 5.7: about 60% of maximal activit, pH 8.5: about 80% of maximal activity, 70°C Pyrococcus abyssi
7 10 pH 7.0: about 60% of maximal activity, pH 10.0: about 60% of maximal activity, 37°C Pyrococcus abyssi

pI Value

Organism Comment pI Value Maximum pI Value
Pyrococcus abyssi isoelectric focusing
-
 4.7