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Literature summary for 6.3.4.16 extracted from
- Role of Cys1327 and Cys1337 in redox sensitivity and allosteric monitoring in human carbamoyl phosphate synthetase (2008), J. Biol. Chem., 284, 5977-5985.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| N-acetylglutamate | the enzyme requires binding of and allosteric activation by N-acetylglutamate for catalytic activity | Homo sapiens |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CPSI, expression of the N-terminally His6-FLAG-tagged enzyme in Schizosaccharomyces pombe from pESP5 | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C1327A | the mutant shows reduced activity compared to the wild-type enzyme, the mutation significantly alters activity at the domain C ATP site, binding of N-acetylglutamate is affected, overview | Homo sapiens |
| C1337A | the mutant shows reduced activity compared to the wild-type enzyme, the mutation significantly alters activity at the domain C ATP site, binding of N-acetylglutamate is affected, overview | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of wild-type and mutant enzymes, overview. The Km of the enzyme for ammonia is 100fold lower than that for CPSs that also use glutamine, EC 6.3.5.5 | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial matrix | - |
Homo sapiens | 5759 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + NH4+ + HCO3- + H2O | Homo sapiens | - |
ADP + phosphate + carbamoyl phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P31327 | gene CPSI | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.43 | - |
purified recombinant tagged mutant C1327A | Homo sapiens |
| 0.47 | - |
purified recombinant tagged mutant C1337A | Homo sapiens |
| 0.86 | - |
purified recombinant tagged wild-type enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + NH4+ + HCO3- + H2O | - |
Homo sapiens | ADP + phosphate + carbamoyl phosphate | - |
? | |
| ATP + NH4+ + HCO3- + H2O | synthesis of carbamoyl phosphate requires coordination of two hCPS active sites with ATP cleavage occurring at duplicated ATP grasp domains B and C | Homo sapiens | ADP + phosphate + carbamoyl phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Carbamoyl-phosphate synthetase I | - |
Homo sapiens |
| CPS1 | - |
Homo sapiens |
| urea-specific CPS | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 30 | assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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