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Literature summary for 6.3.4.16 extracted from
- The carbamoyl-phosphate synthetase of Pyrococcus furiosus is enzymologically and structurally a carbamate kinase (1999), J. Biol. Chem., 274, 16295-16303.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pyrococcus furiosus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| recombinant enzyme | Pyrococcus furiosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + NH3 + CO2 + H2O | Pyrococcus furiosus | the product carbamoylphosphate is used in the biosynethsis of pyrimidines, arginine, and urea | ADP + phosphate + carbamoylphosphate | - |
ir |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Pyrococcus furiosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Pyrococcus furiosus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2 ATP + NH3 + hydrogencarbonate = 2 ADP + phosphate + carbamoyl phosphate | the enzyme is enzymologically and structurally a carbamate kinase, stoichiometry | Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16 | - |
value at 37°C and 60°C | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + carbamate | - |
Pyrococcus furiosus | ADP + carbamoyl phosphate | - |
r | |
| ATP + NH3 + CO2 + H2O | - |
Escherichia coli | ADP + phosphate + carbamoyl phosphate | - |
ir | |
| ATP + NH3 + CO2 + H2O | - |
Pyrococcus furiosus | ADP + phosphate + carbamoyl phosphate | - |
ir | |
| ATP + NH3 + CO2 + H2O | the product carbamoylphosphate is used in the biosynethsis of pyrimidines, arginine, and urea | Pyrococcus furiosus | ADP + phosphate + carbamoylphosphate | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carbamate kinase-like carbamoyl-phosphate synthetase | - |
Pyrococcus furiosus |
| CK-like CPS | - |
Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
extreme thermostable | Pyrococcus furiosus |
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