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Literature summary for 6.3.4.16 extracted from

  • Ramon-Maiques, S.; Marina, A.; Uriarte, M.; Fita, I.; Rubio, V.
    The 1.5 A resolution crystal structure of the carbamate kinase-like carbamoyl phosphate synthetase from the hyperthermophilic Archaeon pyrococcus furiosus, bound to ADP, confirms that this thermostable enzyme is a carbamate kinase, and provides insight into substrate binding and stability in carbamate kinases (2000), J. Mol. Biol., 299, 463-476.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
1.5 A resolution structure of recombinant enzyme Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + NH3 + CO2 + H2O Pyrococcus furiosus the product carbamoyl phosphate is used in the biosynthesis of pyrimidines, arginine, and urea ADP + phosphate + carbamoylphosphate
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ir

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
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-
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Reaction

Reaction Comment Organism Reaction ID
2 ATP + NH3 + hydrogencarbonate = 2 ADP + phosphate + carbamoyl phosphate the enzyme structure is ill-suited for catalysing the characteristic three-step reaction of carbamoyl-phosphate synthetase and supports the view that the carbamate kinase-like carbamoyl-phosphate synthetase is in fact a highly thermostable and very slow carbamate kinase that, in extreme environment of Pyrococcus furiosus, may have the new function of making, rather than using, carbamoyl phosphate Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + carbamate
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Pyrococcus furiosus ADP + carbamoyl phosphate
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r
ATP + NH3 + CO2 + H2O three step reaction Pyrococcus furiosus ADP + phosphate + carbamoyl phosphate
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ir
ATP + NH3 + CO2 + H2O the product carbamoyl phosphate is used in the biosynthesis of pyrimidines, arginine, and urea Pyrococcus furiosus ADP + phosphate + carbamoylphosphate
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ir

Synonyms

Synonyms Comment Organism
carbamate kinase-like carbamoyl-phosphate synthetase
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Pyrococcus furiosus
CK-like CPS
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Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
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the enzyme is extreme thermostable Pyrococcus furiosus