Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetylglutamate | not required as activator | Phycomyces blakesleeanus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NEM | - |
Phycomyces blakesleeanus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.93 | - |
ATP | - |
Phycomyces blakesleeanus | |
6.34 | - |
NH4+ | - |
Phycomyces blakesleeanus | |
10.5 | - |
HCO3- | - |
Phycomyces blakesleeanus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | free Mg2+ required for maximal activity | Phycomyces blakesleeanus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
1 * 130000 + 1 * 55000, SDS-PAGE | Phycomyces blakesleeanus |
130000 | - |
1 * 130000 + 1 * 55000, SDS-PAGE | Phycomyces blakesleeanus |
188000 | - |
gel filtration | Phycomyces blakesleeanus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phycomyces blakesleeanus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Phycomyces blakesleeanus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Phycomyces blakesleeanus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.87 | - |
- |
Phycomyces blakesleeanus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + NH4+ + HCO3- | - |
Phycomyces blakesleeanus | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
2 ATP + NH4+ + HCO3- | ATP in form of MgATP2- | Phycomyces blakesleeanus | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
ATP + NH4+ + CO2 + H2O | no activity with Gln | Phycomyces blakesleeanus | ADP + phosphate + carbamoylphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 130000 + 1 * 55000, SDS-PAGE | Phycomyces blakesleeanus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
38.5 | - |
transition temperature | Phycomyces blakesleeanus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 7.8 | - |
Phycomyces blakesleeanus |