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Literature summary for 6.3.4.16 extracted from

  • Durbecq, V.; Legrain, C.; Roovers, M.; Pierard, A.; Glansdorff, N.
    The carbamate kinase-like carbamoyl phosphate synthetase of the hyperthermophilic archaeon Pyrococcus furiosus, a missing link in the evolution of carbamoyl phosphate biosynthesis (1997), Proc. Natl. Acad. Sci. USA, 94, 12803-12808.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.4.16

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
ADP strong Pyrococcus furiosus
alpha,beta-methyleneATP
-
 Pyrococcus furiosus
AMP
-
 Pyrococcus furiosus
CTP weak Pyrococcus furiosus
ITP weak Pyrococcus furiosus
P1,P5-di-(adenosine-5')pentaphosphate no inhibition by P1,P3-di-(adenosine-5')-pentaphosphate Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 2 * 32000, SDS-PAGE Pyrococcus furiosus
78000 80000 native PAGE, gel filtration Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.67
-
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NH4+ + CO2 + H2O no activity with Gln Pyrococcus furiosus ADP + phosphate + carbamoylphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 3 h, 50% loss of activity Pyrococcus furiosus
100
-
 1 h, 50% loss of activity Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 at 60°C Pyrococcus furiosus
7.8 8 at 37°C Pyrococcus furiosus