General Stability | Organism |
---|---|
glycerol and DTT stabilize during purification | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial matrix | constitutes 22-26% of the total matrix protein | Rattus norvegicus | 5759 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
2 * 120000, SDS-PAGE | Rattus norvegicus |
160000 | 251000 | sedimentation velocity measurement, gel filtration, MW depends on protein concentration and composition of solvent | Rattus norvegicus |
222000 | - |
glycerol or sucrose density gradient centrifugation | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.87 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
4°C, 20% v/v glycerol, 0.1 or 2 mM DTT, 48 h, stable | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NH4+ + CO2 + H2O | - |
Rattus norvegicus | ADP + phosphate + carbamoylphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 120000, SDS-PAGE | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
24 h, 20% v/v glycerol, 2 mM DTT, stable | Rattus norvegicus |
23 | - |
24 h, 20% v/v glycerol, 2 mM DTT, 13% loss of activity | Rattus norvegicus |