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Literature summary for 6.3.4.15 extracted from
- A green fluorescent protein-based assay for high-throughput ligand-binding studies of a mycobacterial biotin protein ligase (2017), Microbiol. Res., 205, 35-39 .
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | development of a high-throughput assay building on the principle of differential scanning fluorimetry of GFP-tagged proteins | Mycobacterium tuberculosis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| codon-optimized expression in Escherichia coli | Mycobacterium tuberculosis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P96884 | - |
- |
| Mycobacterium tuberculosis CDC 1551 | P96884 | - |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BirA | - |
Mycobacterium tuberculosis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
melting temperature does not change significantly in presence of ATP, GTP, CTP or UTP | Mycobacterium tuberculosis |
| 55 | - |
melting temperature, BirA-GFP fusion protein. Enzyme is not able to refold after the first cycle of heat denaturation | Mycobacterium tuberculosis |
| 61 | - |
melting temperature, BirA-GFP fusion protein, presence of 1 mM biotin | Mycobacterium tuberculosis |
| 71 | - |
melting temperature, BirA-GFP fusion protein, presence of both 1 mM biotin and 1 mM ATP | Mycobacterium tuberculosis |
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