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Literature summary for 6.3.4.15 extracted from

  • Delli-Bovi, T.; Spalding, M.; Prigge, S.
    Overexpression of biotin synthase and biotin ligase is required for efficient generation of sulfur-35 labeled biotin in E. coli (2010), BMC Biotechnol., 10, 73.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis production of [35S]-biotin from Na-35SO4 and desthiobiotin with a specific activity of 30.7 Ci/mmol by expression of the biotinylation domain from the Plasmodium falciparum acetyl-CoA carboxylase in Escherichia coli as a biotinylation substrate. Overexpression of the biotin synthase, BioB, and biotin ligase, BirA, increases biotinylation of the biotinylation domain 160fold over basal levels. Biotinylated biotinylation domain is purified by affinity chromatography, and free biotin is liberated using acid hydrolysis Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
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Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum C6S3D7
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