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Literature summary for 6.3.4.15 extracted from

  • Lee, C.K.; Cheong, C.; Jeon, Y.H.
    Substrate recognition characteristics of human holocarboxylase synthetase for biotin ligation (2010), Biochem. Biophys. Res. Commun., 391, 455-460.
    View publication on PubMed
Search for more literature for 6.3.4.15

Cloned(Commentary)

Cloned (Comment) Organism
expression of HCS in Spodoptera frugiperda Sf9 cells and in Hi5 insect cells using the baculovirus transfection system Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant human HCS from Hi5 insect cells by nickel affinity chromatography, anion exchange chromatography, and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + biotin + apo-[acetyl-CoA carboxylase 2] i.e. ACC2, substrate is the recombinantly expressed His-tagged biotinoyl domain, ACC75, of ACC2, comprising residues 891-965. hHCS recognizes the MKM motif, NMR binding study, binding pattern, overview Homo sapiens AMP + diphosphate + acetyl-CoA carboxylase 2
-
 ?
ATP + biotin + apo-[biotin carboxyl carrier protein] i.e. BCCP, substrate is the recombinantly expressed His-tagged biotinoyl domain, BCCP87, of Escherichia coli BCCP, binding pattern, overview Homo sapiens AMP + diphosphate + biotin carboxyl carrier protein
-
 ?

Synonyms

Synonyms Comment Organism
HCS
-
 Homo sapiens
Holocarboxylase synthetase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens