Literature summary for 6.3.4.15 extracted from

Bagautdinov, B.; Matsuura, Y.; Bagautdinova, S.; Kunishima, N.
Protein biotinylation visualized by a complex structure of biotin protein ligase with a substrate (2008), J. Biol. Chem., 283, 14739-14750.

Search for more literature for 6.3.4.15

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, complexed with biotinyl-5'-AMP, biotin, adenosine, or mutant biotin carboxyl carrier protein PhBCCPN76, X-ray diffraction structure determination at 2.0-2.7 A resolution, analysis, and modelling. The non-hydrolyzable intermediate analogue biotinol-5'-AMP does not provide analyzable complex crystals	Pyrococcus horikoshii

Protein Variants

Protein Variants	Comment	Organism
R48A/K111A	site-directed mutagenesis, comparison of the nucleotide binding mode in the double mutant to that in the wild-type enzyme, the active site loop Gly45-Trp53 is disordered in the mutant. Interface charge distribution in the double mutant complex, overview	Pyrococcus horikoshii
R48K	site-directed mutagenesis, comparison of the nucleotide binding mode in the mutant to that in the wild-type enzyme, the active site loop Gly45-Trp53 is disordered in the mutant	Pyrococcus horikoshii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	Pyrococcus horikoshii	-	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	Pyrococcus horikoshii OT-3	-	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	-	strain OT3	-
Pyrococcus horikoshii OT-3	-	strain OT3	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	Pyrococcus horikoshii	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	Pyrococcus horikoshii OT-3	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?
additional information	structural features influencing the reaction mechanism, overview	Pyrococcus horikoshii	?	-	?
additional information	structural features influencing the reaction mechanism, overview	Pyrococcus horikoshii OT-3	?	-	?

Subunits

Subunits	Comment	Organism
More	acetyl-CoA carboxylase biotinyl domain structure analysis, overview	Pyrococcus horikoshii

Synonyms

Synonyms	Comment	Organism
biotin protein ligase	-	Pyrococcus horikoshii
BPL	-	Pyrococcus horikoshii

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Pyrococcus horikoshii

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Release 2023.1 (January 2023)