Literature summary for 6.3.4.15 extracted from

Pendini, N.R.; Bailey, L.M.; Booker, G.W.; Wilce, M.C.; Wallace, J.C.; Polyak, S.W.
Biotin protein ligase from Candida albicans: expression, purification and development of a novel assay (2008), Arch. Biochem. Biophys., 479, 163-169.

Search for more literature for 6.3.4.15

Application

Application	Comment	Organism
drug development	the enzyme is a target for the design of effective antifungal drugs	Candida albicans

Cloned(Commentary)

Cloned (Comment)	Organism
expression of His9-tagged BPL in Saccharomyces cerevisiae strain w303, functional complementation of the conditional lethal birA85 Escherichia coli strain BM4062	Candida albicans

Protein Variants

Protein Variants	Comment	Organism
additional information	disruption of BPL affects important metabolic pathways such as fatty acid biosynthesis and gluconeogenesis	Candida albicans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Candida albicans	5737	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Candida albicans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
73000	-	x * 73000, recombinant His9-tagged BPL, SDS-PAGE	Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	Candida albicans	BPL is an essential enzyme responsible for the activation of biotin-dependent enzymes through the covalent attachment of biotin	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	Candida albicans CBF562	BPL is an essential enzyme responsible for the activation of biotin-dependent enzymes through the covalent attachment of biotin	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?

Organism

Organism	UniProt	Comment	Textmining
Candida albicans	-	-	-
Candida albicans CBF562	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His9-tagged BPL from Saccharomyces cerevisiae strain w303 by nickel affinity and cation exchange chromatography	Candida albicans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.049	-	purified recombinant enzyme	Candida albicans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	BPL is an essential enzyme responsible for the activation of biotin-dependent enzymes through the covalent attachment of biotin	Candida albicans	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	determination of the biotin domains of acetyl-CoA carboxylase, ACC	Candida albicans	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	determination of the biotin domains of the pyruvate carboxylase, PC, of the organism, substrate is peptide fragment CaPC115 as GST-tagged recombinant protein, overview	Candida albicans	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	BPL is an essential enzyme responsible for the activation of biotin-dependent enzymes through the covalent attachment of biotin	Candida albicans CBF562	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	determination of the biotin domains of acetyl-CoA carboxylase, ACC	Candida albicans CBF562	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	determination of the biotin domains of the pyruvate carboxylase, PC, of the organism, substrate is peptide fragment CaPC115 as GST-tagged recombinant protein, overview	Candida albicans CBF562	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?

Subunits

Subunits	Comment	Organism
?	x * 73000, recombinant His9-tagged BPL, SDS-PAGE	Candida albicans

Synonyms

Synonyms	Comment	Organism
biotin protein ligase	-	Candida albicans
BPL	-	Candida albicans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Candida albicans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Candida albicans

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Candida albicans

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Release 2023.1 (January 2023)