Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target for the design of effective antitubercular drugs | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
gene Rv3279c, DNA and amino acid sequence determination and analysis, expression of C-terminally Strep-tagged BirA in Escherichia coli | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, 0.003 ml of 5 mg/ml protein in 2.5 mM desthiobiotin in 20 mM Tris-HCl pH 8.0, 50 mM NaCl, 1 mM PMSF and 1 mM DTT, is mixed with 0.003 ml of reservoir solution containing 12-16% w/v of both PEG 4000 and PEG 8000 in 0.1 M HEPES, pH 7.5, X-ray diffraction structure determination and analysis at 2.8 A resolution | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | Mycobacterium tuberculosis | - |
AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
additional information | Mycobacterium tuberculosis | biotin-dependent acetyl-CoA carboxylases/transcarboxylases are a class of enzymes that, in addition to fatty-acid biosynthesis, are important for gluconeogenesis as well as propionate catabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P96884 | gene Rv3279c | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally Strep-tagged BirA from Escherichia coli | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
Mycobacterium tuberculosis | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
additional information | biotin-dependent acetyl-CoA carboxylases/transcarboxylases are a class of enzymes that, in addition to fatty-acid biosynthesis, are important for gluconeogenesis as well as propionate catabolism | Mycobacterium tuberculosis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
biotin acetyl-CoA carboxylase ligase | - |
Mycobacterium tuberculosis |
BirA | - |
Mycobacterium tuberculosis |
More | the enzyme belongs to the biotin-dependent acetyl-CoA carboxylases/transcarboxylases | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycobacterium tuberculosis | |
biotin | dependent on | Mycobacterium tuberculosis |