Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Sulfurisphaera tokodaii |
Protein Variants | Comment | Organism |
---|---|---|
A43G | catalytic efficiency is 2fold lower than catalytic efficiency of wild-type enzyme | Sulfurisphaera tokodaii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Sulfurisphaera tokodaii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000026 | - |
biotin | pH 8.0, 37°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
0.00044 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C, wild type enzyme | Sulfurisphaera tokodaii | |
0.00058 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
0.018 | - |
ATP | pH 8.0, 37°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
0.063 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 70°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
0.101 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 70°C, wild type enzyme | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | F9VNG7 | - |
- |
Sulfurisphaera tokodaii 7 | F9VNG7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | the enzyme from Sulfolobus tokodaii cannot use wild-type biotin carboxyl carrier protein from Escherichia coli as a substrate | Sulfurisphaera tokodaii | AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein mutant S136M]-biotinyllysine | - |
? | |
ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | the enzyme from Sulfolobus tokodaii cannot use wild-type biotin carboxyl carrier protein from Escherichia coli as a substrate | Sulfurisphaera tokodaii 7 | AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein mutant S136M]-biotinyllysine | - |
? | |
ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | the enzyme from Sulfolobus tokodaii cannot use wild-type biotin carboxyl carrier protein from Escherichia coli as a substrate | Sulfurisphaera tokodaii | AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein]-biotinyllysine | - |
? | |
ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | the enzyme from Sulfolobus tokodaii cannot use wild-type biotin carboxyl carrier protein from Escherichia coli as a substrate | Sulfurisphaera tokodaii 7 | AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein]-biotinyllysine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | biotin-protein-ligase/holo-biotin-carboxyl-carrier-protein complex | Sulfurisphaera tokodaii |
Synonyms | Comment | Organism |
---|---|---|
STK_15250 | locus name | Sulfurisphaera tokodaii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
no denaturation | Sulfurisphaera tokodaii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
0.004 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C, wild type enzyme | Sulfurisphaera tokodaii | |
0.082 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 70°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
0.165 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 70°C, wild type enzyme | Sulfurisphaera tokodaii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
ATP | pH 8.0, 37°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
0.62 | - |
ATP | pH 8.0, 37°C, wild type enzyme | Sulfurisphaera tokodaii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 70°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
1.6 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 70°C, wild type enzyme | Sulfurisphaera tokodaii | |
3.8 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
9.1 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C,wild type enzyme | Sulfurisphaera tokodaii |