Literature summary for 6.3.4.15 extracted from

Bagautdinov, B.; Kuroishi, C.; Sugahara, M.; Kunishima, N.
Crystal structures of biotin protein ligase from Pyrococcus horikoshii OT3 and its complexes: structural basis of biotin activation (2005), J. Mol. Biol., 353, 322-333.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of the enzyme comprising residues 1-125 in Escherichia coli strain BL21	Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant native and selenomethionine-labeled enzyme comprising residues 1-125, sitting drop method, 9.87 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 500 mM NaCl, 0.001 ml protein solution is mixed with 0.001 ml precipitation solution containing 10.5% PEG 2000, 0.1 M acetate-NaOH, pH 5.5, the drop is overlaid with a 1:1 mixture of silicone and paraffin oil allowing slow water evaporation from the drop, 22°C, preliminary X-ray diffraction structure determination and analysis at 1.45-1.6 A resolution	Pyrococcus horikoshii

Crystallization (Comment)

Organism

purified recombinant native and selenomethionine-labeled enzyme comprising residues 1-125, sitting drop method, 9.87 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 500 mM NaCl, 0.001 ml protein solution is mixed with 0.001 ml precipitation solution containing 10.5% PEG 2000, 0.1 M acetate-NaOH, pH 5.5, the drop is overlaid with a 1:1 mixture of silicone and paraffin oil allowing slow water evaporation from the drop, 22°C, preliminary X-ray diffraction structure determination and analysis at 1.45-1.6 A resolution

Pyrococcus horikoshii

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	-	strain OT3	-
Pyrococcus horikoshii OT-3	-	strain OT3	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme comprising residues 1-125 from Escherichia coli strain BL21	Pyrococcus horikoshii

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine	reaction mechanism involving Lys111, formation of a biotinyl-5'-AMP intermediate, active site structure, ligand binding to the active site loop, overview	Pyrococcus horikoshii	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase	Pyrococcus horikoshii	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase	Pyrococcus horikoshii OT-3	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?

Subunits

Subunits	Comment	Organism
dimer	the functional unit is a dimer, a subunit contains a catalytic N-terminal domain and a C-terminal domain, structure analysis	Pyrococcus horikoshii

Synonyms

Synonyms	Comment	Organism
biotin-protein ligase	-	Pyrococcus horikoshii

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Pyrococcus horikoshii