Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is utilized to synthesize site-specific biotinylated proteins via a biotin acceptor peptide tag | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
gene birA, functional expression of N-terminally and C-terminally multiply tagged enzyme in Saccharomyces cerevisiae, low level expression of the surface-displayed enzyme, expression of avitagged-BirA in Saccharomyces cerevisiae, overview | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycerol | inhibits the recombinant enzyme at 10% | Escherichia coli | |
NaCl | inhibits the purified enzyme at 0.1 M, but not the immobilized enzyme in crude extract of recombinant yeast cells | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
avitag-tagged maltose-binding protein | recombinant enzyme | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, recombinant enzyme, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
avitag-tagged maltose-binding protein + biotin + ATP | Escherichia coli | recombinant Saccharomyces cerevisiae cells | maltose-binding protein-avitag-biotin + AMP + diphosphate | - |
? | |
avitagged BirA + biotin + ATP | Escherichia coli | recombinant Saccharomyces cerevisiae cells | BirA-avitag-biotin + AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Saccharomyces cerevisiae, immobilization of the recombinant enzyme | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the immobilized enzyme is slightly less active than the soluble enzyme | Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, immobilized recombinant enzyme, PBS, completely stable for 4 weeks, loss of activity after 5 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
avitag-tagged maltose-binding protein + biotin + ATP | recombinant Saccharomyces cerevisiae cells | Escherichia coli | maltose-binding protein-avitag-biotin + AMP + diphosphate | - |
? | |
avitag-tagged maltose-binding protein + biotin + ATP | recombinant avitag-tagged maltose-binding protein | Escherichia coli | maltose-binding protein-avitag-biotin + AMP + diphosphate | - |
? | |
avitagged BirA + biotin + ATP | recombinant Saccharomyces cerevisiae cells | Escherichia coli | BirA-avitag-biotin + AMP + diphosphate | - |
? | |
avitagged BirA + biotin + biotin + ATP | the recombinant avitagged enzyme biotinylates itself at the avitag sequence | Escherichia coli | BirA-avitag-biotin + AMP + diphosphate | - |
? | |
additional information | the enzyme site-specifically biotinylates proteins via a biotin acceptor peptide tag | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, recombinant enzyme, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
biotin ligase | - |
Escherichia coli |
BirA | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |