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Literature summary for 6.3.4.15 extracted from

  • Landman, A.D.; Desjardins, P.R.; Dakshinamurti, K.
    A simple assay for acetyl-CoA holocarboxylase synthetase (1976), Can. J. Biochem., 54, 813-815.
    View publication on PubMed

Application

Application Comment Organism
analysis biotin-mediated ATP-diphosphate assay which does not require the isolation of the apoenzyme and is simple and convenient for use on a routine assay. The procedure is specifically designed for assay of enzyme from adipose tissue of biotin-deficient rats. W Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
biotin-deficient animals
-

Source Tissue

Source Tissue Comment Organism Textmining
adipose tissue
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
Rattus norvegicus AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
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