Crystallization (Comment) | Organism |
---|---|
in complex with biotinylated biotin carboxyl carrier protein, sitting drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.1 M Bis-Tris (pH 6.5), and 25% (w/v) PEG 3350 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin-carboxyl-carrier protein + HCO3- | Escherichia coli | - |
ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P24182 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity chromatography and gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin-carboxyl-carrier protein + HCO3- | - |
Escherichia coli | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50000, SDS-PAGE | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |