Activating Compound | Comment | Organism | Structure |
---|---|---|---|
biotin | substrate induced synergism, the reaction inpresence of biotin is 1100fold faster than in its absence | Pseudomonas aeruginosa | |
biotin | substrate-induced synergism, the reaction in presence of biotin is 1100fold faster than in its absence | Staphylococcus aureus | |
biotin | substrate-induced synergism, the reaction in presence of biotin is 1100fold faster than in its absence | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
gene accC, expression in Escherichia coli srrain BL21-AI as His-tagged enzyme | Pseudomonas aeruginosa |
gene accC, expression in Escherichia coli strain BL21-AI as His-tagged enzyme | Escherichia coli |
gene accC, overexpression in Escherichia coli strain BL21(DE3) as His-tagged enzyme | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
enzyme in complex with ATP analogues AMP-PNP and ADP-CF2P, hanging drop vapour diffusion, from 0.1 M Bis-Tris, pH 6.5, 0.2 M CaCl2, 45% methylpentanediol, and 10% ethylene glycol or 0.1 M KCl, 3-8% PEG 8000 and 20% ethylene glycol, respectively, X-ray diffraction structure determination and analysis at 2.05 and 2.69 A resolution, respectively, structure modelling | Escherichia coli |
recombinant apoenzyme or enzyme in complex with ATP analogue AMP-PCP, sitting drop vapour diffusion, from 0.1 M HEPES, pH 7.0, 0.2 M MgCl2, 15-20% PEG 3350, and 10% ethylene glycol in the well solution, X-ray diffraction structure determination and analysis at 2.4 A resolution, structure modelling | Pseudomonas aeruginosa |
recombinant enzyme in complex with ATP analogue AMP-PNP, sitting drop vapour diffusion, from 0.2 M KCl, 20% PEG 3350, and 20% ethylene glycol in the well solution, X-ray diffraction structure determination and analysis at 2.10, structure modelling | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
G165V | site-directed mutagenesis, the active site mutant has the residue of the Staphylococcus aureus enzyme and shows increased Km for ATP and 100fold decreased reaction velocity compared to the wild-type enzyme | Escherichia coli |
G166V | site-directed mutagenesis, the active site mutant has the residue of the Staphylococcus aureus enzyme and shows increased Km for ATP and 100fold decreased reaction velocity compared to the wild-type enzyme | Escherichia coli |
K116Q | site-directed mutagenesis, the phosphate binding site mutant has the residue of the Staphylococcus aureus enzyme and shows a 50fold increased Km for ATP compared to the wild-type enzyme | Escherichia coli |
K159Q | site-directed mutagenesis, the active site mutant has the residue of the Staphylococcus aureus enzyme and shows a 90fold higher Km for ATP compared to the wild-type enzyme | Escherichia coli |
R292A | site-directed mutagenesis, the mutant has a Km for ATP similar to the wild-type enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | binding sites structure | Staphylococcus aureus | |
Mg2+ | binding sites structure | Escherichia coli | |
Mg2+ | binding sites structure | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin-carboxyl-carrier protein + CO2 | Staphylococcus aureus | - |
ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? | |
ATP + biotin-carboxyl-carrier protein + CO2 | Escherichia coli | - |
ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? | |
ATP + biotin-carboxyl-carrier protein + CO2 | Pseudomonas aeruginosa | - |
ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? | |
ATP + biotin-carboxyl-carrier protein + CO2 | Staphylococcus aureus Mu50 | - |
ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene accC | - |
Pseudomonas aeruginosa | - |
- |
- |
Staphylococcus aureus | - |
- |
- |
Staphylococcus aureus Mu50 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli srrain BL21-AI by nickel affinity and anion exchange chromatography, and gel filtration | Pseudomonas aeruginosa |
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Staphylococcus aureus |
recombinant His-tagged enzyme from Escherichia coli strain BL21-AI by nickel affinity chromatography and gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin-carboxyl-carrier protein + CO2 | - |
Staphylococcus aureus | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? | |
ATP + biotin-carboxyl-carrier protein + CO2 | - |
Escherichia coli | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? | |
ATP + biotin-carboxyl-carrier protein + CO2 | - |
Pseudomonas aeruginosa | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? | |
ATP + biotin-carboxyl-carrier protein + CO2 | - |
Staphylococcus aureus Mu50 | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? | |
additional information | active site structure, substrate binding and structure-function relationship analysis, overview | Staphylococcus aureus | ? | - |
? | |
additional information | active site structure, substrate binding and structure-function relationship analysis, overview | Escherichia coli | ? | - |
? | |
additional information | active site structure, substrate binding and structure-function relationship analysis, overview | Pseudomonas aeruginosa | ? | - |
? | |
additional information | active site structure, substrate binding and structure-function relationship analysis, overview | Staphylococcus aureus Mu50 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | homodimer | Staphylococcus aureus |
dimer | homodimer | Escherichia coli |
dimer | homodimer | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Staphylococcus aureus | |
ATP | - |
Escherichia coli | |
ATP | - |
Pseudomonas aeruginosa |