Crystallization (Comment) | Organism |
---|---|
sitting-drop vapor diffusion method, crystallization of mutant enzyme E23R and F363A | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E23R | mutant enzyme is monomeric in solution, mutant shows 3fold loss in catalytic activity, mutant enzyme forms the correct dimer at high concentrations. kcat/Km for ATP-hydrolysis is 2.6fold lower than wild-type value | Escherichia coli |
F363A | mutant enzyme forms the correct dimer at high concentrations. kcat/Km for ATP-hydrolysis is identical to wild-type value | Escherichia coli |
R19E | mutant enzyme is monomeric in solution, mutant shows 3fold loss in catalytic activity. kcat/Km for ATP-hydrolysis is 2.5fold lower than wild-type value | Escherichia coli |
R366E | mutant enzyme shows no specific activity at 2.5 mM of enzyme and up to 800 mM of ATP | Escherichia coli |
R401E | mutant enzyme shows no specific activity at 2.5 mM of enzyme and up to 800 mM of ATP | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.093 | - |
ATP | biotin-dependent ATP hydrolysis, mutant enzyme E23R | Escherichia coli | |
0.0948 | - |
ATP | biotin-dependent ATP hydrolysis, mutant enzyme R19E | Escherichia coli | |
0.1049 | - |
ATP | biotin-dependent ATP hydrolysis, mutant enzyme F363A | Escherichia coli | |
0.1152 | - |
ATP | biotin-dependent ATP hydrolysis, wild-type enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P24182 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin-carboxyl-carrier protein + CO2 | - |
Escherichia coli | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | wild-type enzyme, dimerization is not an absolute requirement for the catalytic activity of the Escherichia coli biotin carboxylase subunit | Escherichia coli |
monomer | mutant enzyme R19E and E23R are monomeric in solution | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.071 | - |
ATP | biotin-dependent ATP hydrolysis, mutant enzyme E23R | Escherichia coli | |
0.075 | - |
ATP | biotin-dependent ATP hydrolysis, mutant enzyme R19E | Escherichia coli | |
0.207 | - |
ATP | biotin-dependent ATP hydrolysis, mutant enzyme F363A | Escherichia coli | |
0.228 | - |
ATP | biotin-dependent ATP hydrolysis, wild-type enzyme | Escherichia coli |