Cloned (Comment) | Organism |
---|---|
accC gene, overexpression in Escherichia coli | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
x-ray structure | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E288K | completely inactive mutant, hybrid dimer composed of one subunit having the active site mutation and a second with a wild-type active site: 285fold decreased activity, reduced rate of fatty acid synthesis | Escherichia coli |
K238Q | hybrid dimer composed of one subunit having the active site mutation and a second with a wild-type active site: 94fold decreased activity, reduced rate of fatty acid synthesis | Escherichia coli |
N290A | hybrid dimer composed of one subunit having the active site mutation and a second with a wild-type active site: 28fold decreased activity, reduced rate of fatty acid synthesis | Escherichia coli |
R292A | hybrid dimer composed of one subunit having the active site mutation and a second with a wild-type active site: 39fold decreased activity, reduced rate of fatty acid synthesis | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60.1 | - |
biotin | N290A mutant, homodimer | Escherichia coli | |
64 | - |
biotin | pH 8, 25°C, hybrid dimer K238Q/WT | Escherichia coli | |
123.6 | - |
biotin | R292A mutant, homodimer | Escherichia coli | |
192.7 | - |
biotin | pH 8, 25°C, wild-type enzyme, homodimer | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, both subunits are required for activity and the two subunits must be in communication during enzyme function | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin-carboxyl-carrier protein + HCO3- | Escherichia coli | one component of the multienzyme complex acetyl-CoA carboxylase, catalyzes the ATP-dependent carboxylation of biotin, which is covalently attached to the biotin-carboxyl-carrier protein in vivo, fatty acid synthesis | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
hybrid dimers composed of one subunit having an active site mutation and a second with a wild-type active site | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin + HCO3- | utilizes free biotin as substrate in vitro | Escherichia coli | ADP + phosphate + carboxybiotin | - |
? | |
ATP + biotin-carboxyl-carrier protein + HCO3- | one component of the multienzyme complex acetyl-CoA carboxylase, catalyzes the ATP-dependent carboxylation of biotin, which is covalently attached to the biotin-carboxyl-carrier protein in vivo, fatty acid synthesis | Escherichia coli | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? | |
ATP + biotin-carboxyl-carrier protein + HCO3- | one component of the multienzyme complex acetyl-CoA carboxylase, catalyzes the ATP-dependent carboxylation of biotin, two complete active sites per homodimer | Escherichia coli | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50000, both subunits are required for activity and the two subunits must be in communication during enzyme function | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | ATP-dependent | Escherichia coli |