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Literature summary for 6.3.4.14 extracted from
- Identification of lysine-238 of Escherichia coli biotin carboxylase as an ATP-binding residue (1998), FEBS Lett., 427, 377-380.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli JM109 | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K238A | ATP-binding residue, mutant with much decreased activity, kinetic data | Escherichia coli |
| K238Q | ATP-binding residue, mutant with much decreased activity, kinetic data | Escherichia coli |
| K238R | ATP-binding residue, mutant with much decreased activity, kinetic data | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine diphosphopyridoxal | ATP, ADP, inorganic phosphate and bicarbonate protect against inhibition | Escherichia coli |  |
| additional information | not inhibited by PLP | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
ATP | wild-type BC | Escherichia coli | |
| 1.8 | - |
HCO3- | wild-type BC | Escherichia coli | |
| 3.7 | - |
HCO3- | K238A mutant | Escherichia coli | |
| 3.9 | - |
HCO3- | K238Q mutant | Escherichia coli | |
| 6.8 | - |
HCO3- | K238R mutant | Escherichia coli | |
| 8.6 | - |
ATP | K238Q mutant | Escherichia coli | |
| 13 | - |
ATP | K238R mutant | Escherichia coli | |
| 21 | - |
ATP | K238A mutant | Escherichia coli | |
| 140 | - |
biotin | K238Q mutant | Escherichia coli | |
| 160 | - |
biotin | wild-type BC | Escherichia coli | |
| 180 | - |
biotin | K238A and K238R mutant | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
x * 51000, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | fatty acid synthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| wild-type and mutant, overexpressed BC | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + biotin + HCO3- | - |
Escherichia coli | ADP + phosphate + carboxybiotin | - |
? | |
| ATP + biotin-carboxyl-carrier protein + HCO3- | Lys-238 interacts with the gamma-phosphate group of ATP but is not involved in catalysis, BC is a subunit of acetyl-CoA carboxylase and mediates the carboxylation of enzyme-bound biotin | Escherichia coli | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? | |
| additional information | fatty acid synthesis | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 51000, SDS-PAGE | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | Lys-238 is an ATP-binding residue and interacts with the gamma-phosphate group of ATP, electrostatic interaction | Escherichia coli | |
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