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Literature summary for 6.3.4.14 extracted from
- Protein engineering of pyruvate carboxylase (2004), Eur. J. Biochem., 271, 1391-1400.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of biotin carboxylase domain of pyruvate carboxylase in Escherichia coli | Geobacillus thermodenitrificans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 50.9 | - |
biotin | recombinant biotin carboxylase domain of pyruvate carboxylase | Geobacillus thermodenitrificans |  |
| 62.2 | - |
CO2 | recombinant biotin carboxylase domain of pyruvate carboxylase | Geobacillus thermodenitrificans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus thermodenitrificans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant biotin carboxylase domain of pyruvate carboxylase | Geobacillus thermodenitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + biotin + HCO3- | - |
Geobacillus thermodenitrificans | ADP + phosphate + carboxybiotin | - |
? | |
| ATP + biotin-carboxyl-carrier protein + CO2 | - |
Geobacillus thermodenitrificans | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| biotin carboxylase | domain of pyruvate carboxylase | Geobacillus thermodenitrificans |
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Release 2023.1 (January 2023)
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