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Literature summary for 6.3.4.12 extracted from

  • Pan, X.; Yu, J.; Du, Q.; Zeng, S.; Liu, J.; Jiao, Q.; Zhang, H.
    Efficient synthesis of gamma-glutamyl compounds by co-expression of gamma-glutamylmethylamide synthetase and polyphosphate kinase in engineered Escherichia coli (2020), J. Ind. Microbiol. Biotechnol., 47, 573-583 .
    View publication on PubMed
Search for more literature for 6.3.4.12

Application

Application Comment Organism
synthesis efficient synthesis of gamma-glutamyl compounds by co-expression of gamma-glutamylmethylamide synthetase and polyphosphate kinase in engineered Escherichia coli Methylovorus mays

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Methylovorus mays
Mg2+ activates Methylovorus mays
Mn2+ activates Methylovorus mays
additional information no activation by Zn2+ and Ca2+ Methylovorus mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 SDS-PAGE Methylovorus mays

Organism

Organism UniProt Comment Textmining
Methylovorus mays A9ZPH9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + ethylamine
-
 Methylovorus mays ADP + phosphate + N5-ethyl-L-glutamine
-
 ?

Synonyms

Synonyms Comment Organism
gamma-Glutamylmethylamide synthetase
-
 Methylovorus mays
GMAS
-
 Methylovorus mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Methylovorus mays

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 55 35°C: about 75% of maximal activity, 55°C: about 60% of maximal activity Methylovorus mays

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 3 h, less than 10% loss of activity Methylovorus mays
50
-
 3 h, immediate loss of activity Methylovorus mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Methylovorus mays

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 35% of maximal activity, pH 7.0, about 90% of maximal activity, pH 9.0: about 20% of maximal activity Methylovorus mays
6 10 pH 6.0: about 65% of maximal activity, pH 10.0: about 45% of maximal activity Methylovorus mays

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 24 h, 40% loss of activity Methylovorus mays
9
-
 24 h, complete inactivation Methylovorus mays